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The binding of Pseudomonas aeruginosa pili to glycosphingolipids is a tip‐associated event involving the C‐terminal region of the structural pilin subunit
TLDR
The adherence of the Pseudomonas pilus to glycosphingolipid receptors is a tip-associated phenomenon Involving a tip‐exposed C‐terminal region of the pilin structural subunit.
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The pili of Pseudomonas aeruginosa strains PAK and PAO bind specifically to the carbohydrate sequence βGalNAc(1–4)βGal found in glycosphingolipids asialo‐GM1 and asialo‐GM2
TLDR
The data above demonstrated that the P. aeruginosa pili recognize asialo‐GM1 receptor analogue and that βGalNAc(1–4)βGal disaccharlde is sufficient for binding.
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Structure-function analysis of the adherence-binding domain on the pilin of Pseudomonas aeruginosa strains PAK and KB7.
TLDR
The interactions between the pilin ligand and the A549 cell surface receptors are dependent on the conformation mediated by the disulfide bridge, and the dissimilarities between the PAK and KB7 adhesintopes may suggest that compensatory mutations could occur among different pilin strains so as to allow the Pilin adhesins to interact with the same receptor.
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Antigen‐antibody interactions: Elucidation of the epitope and strain‐specificity of a monoclonal antibody directed against the pilin protein adherence binding domain of Pseudomonas aeruginosa strain K
The C‐terminal region of Pseudomonas aeruginosa strain K (PAK) pilin comprises both an epitope for the strain‐specific monoclonal antibody PK99H, which blocks pilus‐mediated adherence, and the…
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Interaction of the receptor binding domains of Pseudomonas aeruginosa pili strains PAK, PAO, KB7 and P1 to a cross-reactive antibody and receptor analog: implications for synthetic vaccine design.
TLDR
Spectral changes observed in the peptide spectrum with the binding of receptor were similar to those seen for thebinding of antibody, suggesting that the epitope recognizing by the antibody is structurally coincident with the adhesintope recognized by the receptor.
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Interaction between the pili of Pseudomonas aeruginosa PAK and its carbohydrate receptor β-D-GalNAc(1->4) β-D-Gal analogs
TLDR
The results demonstrate that the pili of P. aeruginosa PAK accepts a variety of sugar analogs possessing the sequence beta-D-GalNAc(1-->4)beta- D-Gal, which is the minimal carbohydrate receptor sequence of asialo-GM1.
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Engineering a de novo-designed coiled-coil heterodimerization domain off the rapid detection, purification and characterization of recombinantly expressed peptides and proteins.
TLDR
A unique affinity matrix protein tag system that offers a new alternative to existing peptide or protein fusion tags and demonstrates the utility of a de novo-designed system is developed.
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Production and characterization of a camelid single domain antibody-urease enzyme conjugate for the treatment of cancer.
TLDR
A novel immunoconjugate (L-DOS47) was developed and characterized as a therapeutic agent for tumors expressing CEACAM6 and is being investigated as a potential therapeutic agent in human phase I clinical studies for nonsmall cell lung cancer.
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Engineering a de novo designed coiled-coil heterodimerization domain for the rapid detection, purification and characterization of recombinantly expressed peptides and proteins.
TLDR
A unique affinity matrix protein tag system as a rapid, convenient and sensitive method to detect, purify and characterize newly expressed recombinant peptides or proteins from cell extracts and demonstrates the utility of a de novo-designed system.
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Solution secondary structure of a bacterially expressed peptide from the receptor binding domain of Pseudomonas aeruginosa pili strain PAK: A heteronuclear multidimensional NMR study.
TLDR
The discovery of a double-turn motif in the trans (and cis) recombinant PAK pilin peptide is an extremely important result since the double turn has been implicated as a structural requirement for the recognition of both receptor and antibody.
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