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Intracellular ATP levels are a pivotal determinant of chemoresistance in colon cancer cells.
It is shown that intracellular ATP levels are a core determinant in the development of acquired cross-drug resistance of human colon cancer cells that harbor different genetic backgrounds, and may offer a foundation for new improvements to colon cancer treatment.
Sequence homology and structural similarity between cytochrome b of mitochondrial complex III and the chloroplast b6-f complex: position of the cytochrome b hemes in the membrane.
- W. Widger, W. Cramer, R. Herrmann, A. Trebst
- BiologyProceedings of the National Academy of Sciences…
- 1 February 1984
Calculation of the distribution of hydrophobic residues with a "hydropathy" function that is conserved in this family of proteins implies that the membrane-folding pattern of the 42-kilodalton (kDa) mitochondrial cytochromes involves 8-9 membrane-spanning domains.
Transcription termination factor rho: the site of bicyclomycin inhibition in Escherichia coli.
Findings are consistent with the notion that bicyclomycin expresses its activity by interfering with the polyC-dependent ATPase activity of rho.
Allele-Specific Reprogramming of Cancer Metabolism by the Long Non-coding RNA CCAT2.
The molecular basis for the mode of action of bicyclomycin.
Together, the structural and functional studies demonstrate how 1, a modest rho inhibitor, can disrupt the rho molecular machinery thereby leading to a catastrophic effect caused by the untimely overproduction of proteins not normally expressed constitutively, thusleading to a toxic effect on the cells.
Mutations in the Rho Transcription Termination Factor That Affect RNA Tracking*
It is concluded that the secondary RNA-tracking site consists of amino acids whose putative orientation faces the central hole in Rho and in part reside in two clusters of positively charged residues located above and below the ATP hydrolysis domain.
Axial ligands of chloroplast cytochrome b-559: identification and requirement for a heme-cross-linked polypeptide structure.
Optical, resonance Raman, and electron paramagnetic resonance spectroscopies have been used to characterize the ligands and spin state of the chloroplast cytochrome b-559, showing that two polypeptides, one with 83 residues and a second with 39 residues, most likely constitute the protein of the Cytochrome.
Oxidatively damaged proteins of heart mitochondrial electron transport complexes.
The Antibiotic Bicyclomycin Affects the Secondary RNA Binding Site of Escherichia coli Transcription Termination Factor Rho*
- A. Magyar, Xiangdong Zhang, H. Kohn, W. Widger
- Biology, ChemistryThe Journal of Biological Chemistry
- 11 October 1996
The data suggested that the antibiotic binds to Rho, influencing the secondary RNA binding (tracking) site on Rho and slows the tracking of Rho toward the bound RNA polymerase.
On the structure and function of cytochrome b-559
A sumary of biochemical, biophysical, and molecular biological data is presented which led to the identification of two different polypeptides in the cytochrome b-559 protein, which does not have a precedent among soluble or membrane bound cytochromes.