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Prokaryotes: the unseen majority.

The number of prokaryotes and the total amount of their cellular carbon on earth are estimated to be 4-6 x 10(30) cells and 350-550 Pg of C (1 Pg = 10(15) g), respectively, which is 60-100% of the estimated total carbon in plants.
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Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences

This article proposes rational taxonomic boundaries for high taxa of bacteria and archaea on the basis of 16S rRNA gene sequence identities and suggests a rationale for the circumscription of uncultured taxa that is compatible with the taxonomy of cultured bacteria and Archaea.
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Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology.

An ad hoc committee for the re-evaluation of the species definition in bacteriology met in Gent, Belgium, in February 2002. The committee made various recommendations regarding the species definition…
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Quantitative Comparisons of 16S rRNA Gene Sequence Libraries from Environmental Samples

This method successfully distinguished rRNA gene sequence libraries from soil and bioreactors and correctly failed to find differences between libraries of the same composition.
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Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community.

Two new genera and species are proposed: Microbulbifer hydrolyticus, with type strain IRE-31 (= ATCC 700072), and Marinobacterium georgiense, withtype strain KW-40 (= AtCC 700074).
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Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments.

Genotypic and phenotypic analyses support the creation of two novel species: Silicibacter pomeroyi sp.nov.
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Revised road map to the phylum Firmicutes

The taxonomic road map proposed in Volume 1 and updated and emended in Volume 2 was derived from phylogenetic and principal-component analyses of comprehensive datasets of smallsub unit rRNA sequences, and the structuring of this volume is based on updated and curated databases of processed small-subunit rRNA primary structures.
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Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine environment

The genome sequence of Silicibacter pomeroyi, a member of the marine Roseobacter clade, is described, indicating that this organism relies upon a lithoheterotrophic strategy that uses inorganic compounds to supplement heterotrophy.
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Similarity Analysis of DNAs

All of the procedures discussed in the chapter have been used extensively by bacteriologists and use thermal denaturation, high-performance liquid chromatography (HPLC), or dye-binding fluorimetry.
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Relationship of 16S rRNA sequence similarity to DNA hybridization in prokaryotes.

The relationship between D and S was not significantly different between the prokaryotic domains, and S appeared to be a quasi-molecular clock of approximately constant rate when averaging effects and stochastic factors were taken into account.
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