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Atomic structure of the ectodomain from HIV-1 gp41
TLDR
X-ray crystallography determines the structure of the protease-resistant part of a gp41 ectodomain solubilized with a trimeric GCN4 coiled coil in place of the amino-terminal fusion peptide, and suggests a common mechanism for initiating fusion.
Structural basis for the Golgi membrane recruitment of Sly1p by Sed5p
TLDR
The observed Sly1p‐Sed5p interaction mode indicates how SM proteins can stay associated with the assembling fusion machinery in order to participate in late fusion steps.
Crystal Structure of HIV-1 gp41 Including Both Fusion Peptide and Membrane Proximal External Regions
TLDR
The crystal structure at 2 Å resolution of the complete extracellular domain of gp41 lacking the fusion peptide and the cystein-linked loop is reported and it is proposed that this framework has important implications for membrane bending on the viral membrane, which is required for fusion and could provide a platform for epitope and lipid bilayer recognition for broadly neutralizing gp41 antibodies.
Helical Structures of ESCRT-III Are Disassembled by VPS4
TLDR
It is found that the ESCRT-III proteins CHMP2A and CHMP3 (charged multivesicular body proteins 2A and 3) could assemble in vitro into helical tubular structures that could assemble within the neck of an inwardly budding vesicle, catalyzing late steps in budding under the control of VPS4.
Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals
TLDR
This study reveals that by using appropriate screening methods, a large proportion of memory B cells can be isolated that produce mAbs with HIV-1 neutralizing activity, and three of these mAbs show unusual breadth of neutralization and therefore add to the current panel of HIV- 1 neutralizing antibodies with potential for passive protection and template-based vaccine design.
In vitro dissection of the membrane and RNP binding activities of influenza virus M1 protein.
TLDR
The structure/function of influenza virus M1 will be compared with that of the Ebola virus matrix protein, VP40, and several independent experimental approaches indicate that in vitro transcription inhibition occurs through polymerisation/aggregation of M 1 onto RNP, or of M1 onto M1 already bound to RNP.
Oligomerization of type III secretion proteins PopB and PopD precedes pore formation in Pseudomonas
TLDR
Formation of metastable oligomers precedes membrane association and ring generation in the formation of the Pseudomonas translocon, a mechanism which may be similar for other pathogens that employ type III secretion systems.
Lassa Virus Z Protein Is a Matrix Protein Sufficient for the Release of Virus-Like Particles
TLDR
It is demonstrated that the Lassa virus Z protein is abundant in viral particles, is strongly membrane associated, is sufficient in the absence of all other viral proteins to release enveloped particles, and contains two late domains necessary for the release of virus-like particles.
Crystal Structure of the Rabies Virus Nucleoprotein-RNA Complex
TLDR
RNA sequestering by nucleoproteins is likely a common mechanism used by negative-strand RNA viruses to protect their genomes from the innate immune response directed against viral RNA in human host cells at certain stages of an infectious cycle.
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