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Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
The theoretical basis and some practical guidelines for simple, rigorous analysis of FPR experiments are presented and some model experiments on aqueous solutions of rhodamine 6G are described. Expand
Precise nanometer localization analysis for individual fluorescent probes.
A localization algorithm motivated from least-squares fitting theory is constructed and tested both on image stacks of 30-nm fluorescent beads and on computer-generated images (Monte Carlo simulations), and results show good agreement with the derived precision equation. Expand
Two-photon laser scanning fluorescence microscopy.
The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation. Expand
Nonlinear magic: multiphoton microscopy in the biosciences
Multiphoton microscopy has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals and its use is now increasing exponentially. Expand
Thermodynamic Fluctuations in a Reacting System-Measurement by Fluorescence Correlation Spectroscopy
The temporal correlations of thermodynamic concentration fluctuations have been measured in a chemically reactive system at equilibrium by observing fluctuations of the fluorescence of a reactionExpand
Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation
Applications involving a range of intrinsic molecules and molecular assemblies that enable direct visualization of tissue morphology, cell metabolism, and disease states such as Alzheimer's disease and cancer are compiled and demonstrated. Expand
Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension
It is shown that high-resolution fluorescence imaging using two dyes preferentially labelling different fluid phases directly provides a correlation between domain composition and local membrane curvature, which is able to provide experimental estimates of boundary tension between fluid bilayer domains. Expand
Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm
Measurements of two-photon fluorescence excitation (TPE) spectra are presented for 11 common molecular fluorophores in the excitation wavelength range 690 nm < λ < 1050 nm. Results of excitation byExpand
Zero-Mode Waveguides for Single-Molecule Analysis at High Concentrations
It is shown that arrays of zero-mode waveguides consisting of subwavelength holes in a metal film provide a simple and highly parallel means for studying single-molecule dynamics at micromolar concentrations with microsecond temporal resolution. Expand
Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation.
While both excitation alternatives work well for intracellular FCS in thin preparations, 2PE can substantially improve signal quality in turbid preparations like plant cells and deep cell layers in tissue, thereby preserving long-term signal acquisition. Expand