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Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation.
A method is described for determining whether particular monoclonal antibodies are specific for carbohydrate or non-carbohydrate antigenic determinants. In a model system consisting of the Lewis aExpand
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Determination of plasma membrane lipid mass and composition in cultured Chinese hamster ovary cells using high gradient magnetic affinity chromatography.
We have utilized wheat germ agglutinin conjugated to iron/dextran particles in conjunction with high gradient magnetic affinity chromatography (HIMAC) to prepare plasma membranes from cultured cells.Expand
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MUC1 splice variants in human ocular surface tissues: possible differences between dry eye patients and normal controls.
Mucins are highly glycosylated proteins that are vital to the maintenance of healthy epithelial surfaces including the ocular surface. Mucins act as lubricants, protectants, and mediators of signalExpand
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Transport of newly synthesized glucosylceramide to the plasma membrane by a non-Golgi pathway.
High-gradient magnetic affinity chromatography (HIMAC) has been used to obtain highly enriched plasma membranes, free of intracellular membrane contaminants, from cultured Chinese hamster ovary (CHO)Expand
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Staphylococcal protein A binding to the Fab fragments of mouse monoclonal antibodies.
Two mouse IgG1 monoclonal antibodies specific for the Lewis(a) human blood group antigen were purified on protein A-Sepharose by using buffers of decreasing pH for elution. Unlike other IgG1Expand
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β1,4-N-Acetylgalactosaminyltransferase (GM2 Synthase) Is Released from Golgi Membranes as a Neuraminidase-sensitive, Disulfide-bonded Dimer by a Cathepsin D-like Protease*
Many Golgi membrane-bound glycosyltransferases are released from cells in a soluble form. To characterize this release process, we stably transfected Chinese hamster ovary cells with three mycExpand
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Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-N-glycosyl type II chain.
Two monoclonal antibodies directed to the Type II carbohydrate chain of glycosphingolipids have been prepared by the murine hybridoma technique. Their reactivity was determined by liposome lysis,Expand
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Isoantigenic expression of Forssman glycolipid in human gastric and colonic mucosa: its possible identity with "A-like antigen" in human cancer.
The heterogenetic Forssman antigen is a glycosphingolipid, a ceramide pentasaccharide with the structure GalNAcalpha1-->3GalNAcbeta1-->3Galalpha1-->4Galbeta1-->4Glc-->ceramide. Forssman-positiveExpand
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Therapy of mouse lymphoma with monoclonal antibodies to glycolipid: selection of low antigenic variants in vivo.
Growth of mouse lymphoma L5178Y, which contains large quantitites of the gangliotriosylceramide (GgOs3Cer), in DBA/2 mice was suppressed by passive immunization with monoclonal immunoglobulin G3Expand
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Adenylate cyclase toxins from Bacillus anthracis and Bordetella pertussis. Different processes for interaction with and entry into target cells.
Adenylate cyclase (AC) toxins produced by Bacillus anthracis and Bordetella pertussis were compared for their ability to interact with and intoxicate Chinese hamster ovary cells. At 30 degrees C,Expand
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