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Spin-labeled Neurospora mitochondria.
Use of spin labels to evaluate effects of cold shock and osmolality on sperm.
Sperm from 6 species were ranked in terms of susceptibility to membrane damage caused by rapid cooling to 0/sup 0/C, and the integrity of bull and ram sperm membranes was destroyed by the rapid cooling; BHT protected membranes of these spermatozoa from cold-induced lysis.
Viscosity of Cellular Protoplasm
The protoplasmic viscosity was studied using a small spin label having high permeability and broad solubility properties and nickel chloride as an extracellular spin-subtracting agent to localize signal inside cells, suggesting that lateral diffusion in biological membranes is important to cell function.
Use of spin labels and electron spin resonance spectroscopy to characterize membranes of bovine sperm: effect of butylated hydroxytoluene and cold shock.
Spin label probes were used in conjunction with measurements of metabolic rate and electron microscopy to characterize bovine sperm membranes to quantify changes in sperm membranes occurring during the epididymal maturation of sperm and subsequent events within the female tract leading to fertilization.
A fluorescence enhancement assay of cell fusion.
Two probes were synthesized which consist of fluorescent molecules conjugated to saturated hydrocarbon chains, 18 carbons long, to ensure their localization into cellular membranes to investigate resonance energy transfer and the possible application of this assay to other types of membrane fusion.
Inactivation of the Enveloped Bacteriophage ø6 by Butylated Hydroxytoluene and Butylated Hydroxyanisole
Butylated hydroxytoluene (BHT) is a potent inactivator of the enveloped bacterial virus ø6 at concentrations as low as 3 × 10−5 M and a structurally related molecule, butylated hydroxyanisole (BHA), was also found to inactivate ø 6 virus, but higher concentrations were required than with BHT.
Ultraviolet mutagenesis and its repair in an Escherichia coli strain containing a nonsense codon.
It is proposed that theDimer serves as template during some aspects of repair replication and at the time of replication the C in the dimer directs the insertion of A in the complementary strand.
Spin-label studies on the aqueous regions of phospholipid multilayers.
Water-soluble spin labels were used to study dimyristoyllecithin (DML) phospholipid multilayers to show the usefulness of this approach in studying solute behavior when freezing occurs and potential studies involving aqueous regions of cytoplasm are considered.