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Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men.
TLDR
The freeze-thawing procedure significantly affects chromatin structure and sperm morphology, especially in the head and the tail regions, and this may explain the lower fertilization rate and IVF/ICSI outcome when frozen-thawed spermatozoa are used. Expand
The effect of chromatin condensation (aniline blue staining) and morphology (strict criteria) of human spermatozoa on fertilization, cleavage and pregnancy rates in an intracytoplasmic sperm
TLDR
It is demonstrated that neither chromatin condensation (Aniline Blue staining) nor morphology could assess the fertilization potential, cleavage and pregnancy rate in an ICSI programme. Expand
Comparison of sperm preparation methods: effect on chromatin and morphology recovery rates and their consequences on the clinical outcome after in vitro fertilization embryo transfer.
TLDR
PureSperm gradient centrifugation yields a higher percentage of morphologically normal spermatozoa than shown in traditional swim-up or glass-wool filtration, which should be recommended as the first choice for semen preparation for Intracytoplasmic sperm injection technique as the natural selection is bypassed. Expand
Association between sperm cell chromatin condensation, morphology based on strict criteria, and fertilization, cleavage and pregnancy rates in an IVF program
TLDR
The fertilization and pregnancy rates correlate significantly with morphologically normal spermatozoa. Expand
Comparison of reactive oxygen species concentration in seminal plasma and semen parameters in partners of pregnant and non-pregnant patients after IVF/ICSI.
TLDR
A negative correlation between the ROS concentration in seminal plasma and fertilization rate in both IVF/ICSI programmes was shown and an inverse correlation was found between TUNEL, vitality, and membrane integrity. Expand
Comparison between chromatin condensation and morphology from testis biopsy extracted and ejaculated spermatozoa and their relationship to ICSI outcome.
TLDR
The fertilization capability and the embryo quality obtained using testis biopsy extracted spermatozoa is not influenced by chromatin condensation and sperm morphology in testicular sperm extraction (TESE)-ICSI programmes. Expand
Reactive oxygen species, total antioxidant concentration of seminal plasma and their effect on sperm parameters and outcome of IVF/ICSI patients
TLDR
ROS concentration in seminal plasma affects the quality of spermatozoa but does not affect the fertilization rate in IVF/ICSI cycles, and TAS concentration was comparable between the two groups. Expand
Comparison between human sperm preservation medium and TEST-yolk buffer on protecting chromatin and morphology integrity of human spermatozoa in fertile and subfertile men after freeze-thawing
TLDR
TYB could be recommended as a first choice cryoprotectant for semen preservation in order to avoid extra chromatin structure damage and morphology alterations of spermatozoa not only for patients pursuing assisted reproduction, but also for donor samples. Expand
Fibroblast Growth Factor (FGF), Intracellular Adhesion Molecule (sICAM‐1) Level in Serum and Follicular Fluid of Infertile Women with Polycystic Ovarian Syndrome, Endometriosis and Tubal Damage, and
PROBLEM: The objective of this study was to determine the concentration of fibroblast growth factor (FGF) and soluble intracellular adhesions molecule (sICAM‐1) in serum and follicular fluid (FF) ofExpand
PREDICTIVE VALUE OF SPERM CHROMATIN CONDENSATION (ANILINE BLUE STAINING) IN THE ASSESSMENT OF MALE FERTILITY
TLDR
It is suggested that chromatin condensation constitutes a valuable parameter in the assessment of male fertility, completely independent of conventional sperm parameters, and the inclusion of chromatincondensation to routine laboratory investigations of semen prior to assisted reproduction is strongly recommended. Expand
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