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Real-Time Imaging of Fluorescent Flagellar Filaments
A simple procedure for fluorescently labeling cells and filaments that allows recording their motion in real time with an inexpensive video camera and an ordinary fluorescence microscope with mercury-arc or strobed laser illumination is found.
Dimensionality and Dynamics in the Behavior of C. elegans
It is shown that the space of shapes adopted by the nematode Caenorhabditis elegans is low dimensional, with just four dimensions accounting for 95% of the shape variance, and Stimulus-dependent correlations among the different modes suggest that one can generate more reliable behaviors by synchronizing stimuli to the state of the worm in shape space.
An Imbalancing Act: Gap Junctions Reduce the Backward Motor Circuit Activity to Bias C. elegans for Forward Locomotion
Thermotaxis in Caenorhabditis elegans Analyzed by Measuring Responses to Defined Thermal Stimuli
Analysis of the movements of individual worms responding to defined thermal gradients found evidence for a mechanism for migration down Thermal gradients that is active at temperatures above the cultivation temperature, and a mechanisms for isothermal tracking that isactive near the cultivationTemperature.
A Comprehensive Genetic Characterization of Bacterial Motility
A powerful experimental framework is developed that combines competitive selection and microarray-based genetic footprinting to comprehensively reveal the genetic basis of bacterial behaviors and uncovers genome-wide epistatic interactions through comprehensive analyses of double-mutant phenotypes.
Torque-generating units of the flagellar motor of Escherichia coli have a high duty ratio
By inducing expression of MotA in motA- cells under conditions of low viscous load, it is shown that the limiting speed of the motor is independent of the number of units: at vanishing load, one unit turns the motor as rapidly as many.
Enhanced Caenorhabditis elegans Locomotion in a Structured Microfluidic Environment
- Sungsu Park, Hyejin Hwang, Seong-Won Nam, F. Martinez, R. Austin, W. Ryu
- Biology, EngineeringPloS one
- 25 June 2008
These results show that the microstructure can be used as a behavioral screen for mechanosensory and uncoordinated mutants, and it is likely that worms use mechanosensation in the movement and navigation through heterogeneous environments.
Flagellar determinants of bacterial sensitivity to chi-phage.
- A. Samuel, T. Pitta, W. Ryu, P. Danese, E. C. Leung, H. Berg
- BiologyProceedings of the National Academy of Sciences…
- 17 August 1999
Evidence is provided for a "nut and bolt" model for translocation of phage along the filament: the tail fiber of chi fits the grooves formed by helical rows of flagellin monomers, and activeFlagellar rotation forces the phage to follow the groove as a nut follows the threads of a bolt.
Force and Velocity of Mycoplasma mobile Gliding
The gliding speed depended on temperature, but the maximum force did not, suggesting that the mechanism is composed of at least two steps, one that generates force and another that allows displacement.