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Gold nanoparticle-enhanced target (AuNPET) as universal solution for laser desorption/ionization mass spectrometry analysis and imaging of low molecular weight compounds.
A durable surface of cationic gold nanoparticles (AuNPs), covering commercial and custom-made MALDI targets, along with characterization of the nanoparticle surface properties and examples of the use in MS analyses and MS imaging of low molecular weight organic compounds are described. Expand
Phosphorylation of basic amino acid residues in proteins: important but easily missed.
An overview of the current knowledge on phosphorylation of protein basic amino-acid residues takes into consideration its proved or possible roles in cell functioning and specific requirements of studies on acid-labile protein phosphorylations are indicated. Expand
Regulation of thymidylate synthetase in mouse leukemia cells (L1210).
Results of isotope-dilution analysis of the release of tritium from [5-3H]deoxyuridine in the intact cells indicated that thymidine added to the medium caused strong inhibition of thymidylate synthetase, the most likely limiting step in this pathway. Expand
Silver nanostructures in laser desorption/ionization mass spectrometry and mass spectrometry imaging.
An overview of the literature on silver nanomaterials as non-conventional desorption and ionization promoters in LDI-MS and mass spectrometry imaging is provided. Expand
2-Thio derivatives of dUrd and 5-fluoro-dUrd and their 5'-monophosphates: synthesis, interaction with tumor thymidylate synthase, and in vitro antitumor activity.
The beta-anomer 1 exhibited antitumor activity in a mouse leukemic cell line L5178Y (IC50 approximately 10(-6) M), hence 40-100-fold weaker than 5-fluoro-dUrd, but exhibited at least 10-fold higher selectivity with respect to the tumor cells than the beta-Anomer 1. Expand
Studies on the interaction with thymidylate synthase of analogues of 2'-deoxyuridine-5'-phosphate and 5-fluoro-2'-deoxyuridine-5'-phosphate with modified phosphate groups.
5-FdUMP and its analogues exhibited differing interactions with two binding sites on the enzyme molecule, consistent with cooperativity of binding, and a convenient procedure is described for the synthesis of 5-fluoro-2'-deoxyuridine-5'-methylphosphonate, applicable also to the preparation of other 5'-methyl phosphonate analogues. Expand
Functional gene expression profile underlying methotrexate-induced senescence in human colon cancer cells
Cellular functions accompanying establishment of premature senescence in methotrexate-treated human colon cancer C85 cells are deciphered in the present study from validated competitive expressionExpand
Electron capture dissociation mass spectrometric analysis of lysine-phosphorylated peptides
Two MS fragmentation methods, ECD (electron-capture dissociation) and CID (collision-induced dissociation), were tested as tools for analysis of N-phosphorylation of three model peptides, finding the ECD method useful for the localization of phosphorylation sites in unstable lysine-ph phosphorylated peptides. Expand
Structure and conformation of N4-hydroxycytosine and N4-hydroxy-5-fluorocytosine. A theoretical ab initio study.
The results presented in this paper suggest that the syn-imino and anti-iminos forms can be treated as two structural isomers that do not interconvert at temperatures relevant to biochemical conditions. Expand
Phosphorylation of thymidylate synthase from various sources by human protein kinase CK2 and its catalytic subunits.
Human TS exhibits unaltered K(m) values for dUMP and N(5,10)-methylenetetrahydrofolate, but a 50% lower turnover number, pointing to a strong influence of Ser(124) phosphorylation on its catalytic efficiency. Expand