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Histological Effects of Calcium Chloride in Stored Apples
Fruits pressure-infiltrated with 6% CaCl2 exhibited the greatest amount of flattened epidermal cells and hypodermal cavities, and all tissues, including the cuticles, were stained magenta red, indicating a possible chemical alteration of the cuticle and the underlying tissue by Ca.
Staining paraffin extracted, alcohol rinsed and air dried plant tissue with an aqueous mixture of three dyes.
A staining solution containing alcian blue 8GX, Bismarck brown Y and safranin O was prepared with 0.1 M sodium acetate buffer and structures could be distinguished within 30 min as follows: nonlignified cell walls, blue; lignifiedcell walls, nuclei and chloroplasts, red; and cuticle, brown or yellow-brown.