• Publications
  • Influence
Hypochlorous acid interactions with thiols, nucleotides, DNA, and other biological substrates.
  • W. Prütz
  • Chemistry, Medicine
  • Archives of biochemistry and biophysics
  • 1 August 1996
HOC1-induced one-electron oxidation of Fe(CN)(6)4- was used as a reference reaction to investigate the stoichiometry of interaction of HOCl with a variety of biological substrates. GSH and GSSG wereExpand
  • 240
  • 14
Reactions of nitrogen dioxide in aqueous model systems: oxidation of tyrosine units in peptides and proteins.
By application of pulse radiolysis it was demonstrated that nitrogen dioxide (NO2.) oxidizes Gly-Tyr in aqueous solution with a strongly pH-dependent rate constant (k6 = 3.2 X 10(5) M-1 S-1 at pH 7.5Expand
  • 288
  • 9
Interactions of hypochlorous acid with pyrimidine nucleotides, and secondary reactions of chlorinated pyrimidines with GSH, NADH, and other substrates.
  • W. Prütz
  • Chemistry, Medicine
  • Archives of biochemistry and biophysics
  • 1998
HOCl-induced chlorination of pyrimidine nucleotides, PyNH, strikingly depends on the nature of the available chlorine acceptor group. For CMP, with an -NH2 group as acceptor, the reaction is slow andExpand
  • 103
  • 6
On the irreversible destruction of reduced nicotinamide nucleotides by hypohalous acids.
Degradation of the reduced pyridine nucleotides NMNH and NADH by HOCl involves two distinct stages: a fast reaction, k = 4.2 x 10(5) M(-1) s(-1), leads to generation of stable pyridine productsExpand
  • 54
  • 3
Consecutive halogen transfer between various functional groups induced by reaction of hypohalous acids: NADH oxidation by halogenated amide groups.
  • W. Prütz
  • Chemistry, Medicine
  • Archives of biochemistry and biophysics
  • 1 November 1999
Cyclic dipeptides (c-Gly(2), c-Ser(2), c-Gly-Phe, etc.) were used as simple protein models to investigate the HOCl-induced generation and reactivity of chlorinated amide groups. The pH dependence ofExpand
  • 39
  • 3
The glutathione free radical equilibrium, GS. + GS−⇌ GSS.−G, mediating electron transfer to FE(III) -cytochrome c
Abstract The GS.+GS− ⇌ GSS.− G equilibrium (1) was reinvestigated as a function of pH and ionic strength, using pulse radiolysis to oxidize GSH to GS.. All radicals formed by water radiolysis can beExpand
  • 33
  • 2
Reactions of hypochlorous acid with biological substrates are activated catalytically by tertiary amines.
  • W. Prütz
  • Chemistry, Medicine
  • Archives of biochemistry and biophysics
  • 15 September 1998
The activation of reactions of HOCl with a variety of model substrates by tertiary amines was investigated spectroscopically by tandem-mix and stopped-flow techniques. HOCl-induced chlorination ofExpand
  • 56
  • 2
Oxidation of NADH by chloramines and chloramides and its activation by iodide and by tertiary amines.
Irreversible oxidation of reduced nicotinamide nucleotides by neutrophil-derived halogen oxidants (HOCl, chloramines, HOBr, etc.) is likely to be a highly lethal process, because of the essentialExpand
  • 16
  • 2
Charge transfer between tryptophan and tyrosine in proteins
Abstract With numerous proteins, most of which are not involved in oxidation reduction reactions, azide radicals have been found to react with tryptophan units to give Trp. radicals. The Trp.Expand
  • 109
  • 1
Charge transfer in peptides: Intramolecular radical transformations involving methionine, tryptophan and tyrosine
Abstract An efficient intramolecular transfer of electron deficiency from tryptophan (TrpH) to tyrosine (TyrOH) was observed in numerous synthetic and natural peptides in aqueous solutions. TheExpand
  • 96
  • 1