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High‐efficiency gene targeting in Schizosaccharomyces pombe using a modular, PCR‐based approach with long tracts of flanking homology
Bähler et al.(1998) recently described a PCR‐based system for the deletion, tagging and overexpression of endogenous genes in the fission yeast Schizosaccharomyces pombe. A small set of PCR primers…
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Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M26 meiotic recombination hotspot in Schizosaccharomyces pombe.
Homologous recombination hotspots increase the frequency of recombination in nearby DNA. The M26 hotspot in the ade6 gene of Schizosaccharomyces pombe is a meiotic hotspot with a discrete, cis-acting…
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Regulation of the Mts1-Mts2-Dependentade6-M26 Meiotic Recombination Hot Spot and Developmental Decisions by the Spc1 Mitogen-Activated Protein Kinase of Fission Yeast
TLDR
It is reported that the Mts proteins are key effectors of at least two distinct classes of developmental decisions regulated by the mitogen-activated protein (MAP) kinase cascade.
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Roles of histone acetylation and chromatin remodeling factor in a meiotic recombination hotspot
TLDR
The results suggest that HATs and ADCRs cooperatively alter local chromatin structure, as in selective transcription activation, to activate meiotic recombination at M26 in a site‐specific manner.
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A heteromeric protein that binds to a meiotic homologous recombination hot spot: correlation of binding and hot spot activity.
TLDR
Binding in vitro strictly correlates with hot spot activity in vivo for numerous single base pair substitutions in the vicinity of the M26 site, indicating that Mts1/Mts2 activates the M 26 site and promotes a rate-limiting step of meiotic recombination.
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A CRISPR-based approach for proteomic analysis of a single genomic locus
TLDR
The CRISPR-ChAP-MS approach revealed changes in the local epiproteome of a promoter during activation of transcription, which has broad applications for discovering molecular components and dynamic regulation of any in vivo activity at a given chromosomal location.
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Discrete DNA sites regulate global distribution of meiotic recombination.
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Meiotic chromosome segregation mutants identified by insertional mutagenesis of fission yeast Schizosaccharomyces pombe; tandem-repeat, single-site integrations.
TLDR
Molecular and real-time PCR analyses revealed the presence of multiple, truncated copies of ura4+ at each integration site, indicating that electroporation-mediated insertional mutagenesis in S.pombe is preceded by exonucleolytic processing and concatomerization of the transforming DNA.
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Meiotic Recombination Hotspots of Fission Yeast Are Directed to Loci that Express Non-Coding RNA
TLDR
Meiotic DSB hotspots are directed to loci that express polyadenylated ncRNAs, which reveals an unexpected, possibly unitary mechanism for what directs meiotic recombination to hotspots and reveals a likely biological function for enigmatic nc RNAs.
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Chromatin-mediated regulators of meiotic recombination revealed by proteomics of a recombination hotspot
TLDR
Findings indicate that a remarkably diverse collection of chromatin remodeling factors and histone PTMs participate in designating where meiotic recombination occurs in the genome, and they provide new insight into molecular mechanisms of the process.
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