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Combined in silico modeling and metabolomics analysis to characterize fed‐batch CHO cell culture
The increasing demand for recombinant therapeutic proteins highlights the need to constantly improve the efficiency and yield of these biopharmaceutical products from mammalian cells, which is fullyExpand
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Metabolomics-driven approach for the improvement of Chinese hamster ovary cell growth: overexpression of malate dehydrogenase II.
We have established a liquid chromatography-mass spectrometry based metabolomics platform to identify extracellular metabolites in the medium of recombinant Chinese hamster ovary (CHO) fed-batchExpand
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LC‐MS‐based metabolic characterization of high monoclonal antibody‐producing Chinese hamster ovary cells
The selection of suitable mammalian cell lines with high specific productivities is a crucial aspect of large‐scale recombinant protein production. This study utilizes a metabolomics approach toExpand
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Metabolomics profiling of extracellular metabolites in recombinant Chinese Hamster Ovary fed-batch culture.
A metabolomics-based approach was used to time profile extracellular metabolites in duplicate fed-batch bioreactor cultures of recombinant Chinese Hamster Ovary (CHO) cells producing monoclonal IgGExpand
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Advances in sample preparation and analytical techniques for lipidomics study of clinical samples
Abstract Lipids play multiple key roles in biological systems, including acting as secondary energy reserves, being components of cellular membranes and participating in cell-signaling pathways. As aExpand
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Metabolomics-based identification of apoptosis-inducing metabolites in recombinant fed-batch CHO culture media.
A liquid chromatography-mass spectrometry (LC-MS) based metabolomics platform was previously established to identify and profile extracellular metabolites in culture media of mammalian cells. ThisExpand
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Development and application of microwave-assisted extraction technique in biological sample preparation for small molecule analysis
Microwave-assisted extraction (MAE) has emerged as an efficient extraction technique for various kinds of biological samples due to its low usage of extraction solvents and shorter extraction time.Expand
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Enhanced IFNγ production in adenosine‐treated CHOCells: A mechanistic study
Adenosine causes growth arrest in recombinant mammalian cell cultures, which results in enhanced productivity of the recombinant protein. Adenosine is also known to increase intracellular ATP levelExpand
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