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A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada).
The emergence of this usually tropical pathogen on Vancouver Island highlights the changing distribution of this genotype and emphasizes the importance of an ongoing collaborative effort to monitor the global epidemiology of this yeast.
Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates
- W. Meyer, A. Castañeda, S. Jackson, M. Huynh, E. Castañeda
- BiologyEmerging infectious diseases
- 1 February 2003
To acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase gene restriction fragment length polymorphsm analysis.
DNA Fingerprinting in Plants and Fungi
A comparison of Methods for Detecting Genetic Variation and Applications of DNA Fingerprinting in Plants and Fungi and the Spectrum of DNA Typing Strategies is presented.
Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii.
This communication describes the consensus multi-locus typing scheme established by the Cryptococcal Working Group I (Genotyping of Cryptococcus neoformans and C. gattii) of the International Society…
Cryptococcus gattii Infections
Preliminary evidence suggests that C. gattii VGII causes severe lung disease and death without dissemination, whereas C. neoformans disseminates readily to the central nervous system (CNS) and causes death from meningoencephalitis.
Hybrid genotypes in the pathogenic yeast Cryptococcus neoformans.
The authors propose the existence of two species, C. neoformans (Sanfelice) Vuillemin and C. bacillisporus Kwon-Chung, which differ in geographical distribution, serotypes and ecological origin, and within each species three AFLP genotypes occur.
Survey of simple sequence repeats in completed fungal genomes.
The analysis showed that the relative abundance of SSR in fungi is low compared with the human genome and that longer SSRs in fungi are rare, and N. crassa, the largest genome analyzed had the highest relative abundance.
Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans
- W. Meyer, T. Mitchell, E. Freedman, R. Vilgalys
- BiologyJournal of clinical microbiology
- 1 September 1993
Amplification of polymorphic DNA patterns by PCR with these primers offers several advantages over classical DNA fingerprinting techniques, appears to be more reliable than other PCR-based methods for detecting polymorphIC DNA, such as analysis of random-amplified polymorphicDNA, and should be applicable to many other organisms.
Molecular typing of global isolates of Cryptococcus neoformans var. neoformans by polymerase chain reaction fingerprinting and randomly amplified polymorphic DNA — a pilot study to standardize…
All US isolates could be differentiated by a unique, strain‐specific PCR fingerprint or RAPD pattern in contrast to most of the non‐US isolates, which showed a substantially higher degree of genetic homogeneity, with some clonality, in different parts of the world.
International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database--the quality controlled standard tool for routine identification of human and animal pathogenic fungi.
An international consortium of medical mycology laboratories was formed aiming to establish a quality controlled ITS database, containing 2800 ITS sequences representing 421 fungal species, providing the medical community with a freely accessible tool to rapidly and reliably identify most agents of mycoses.