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Storage of boar semen.
The problems, aspects and methods of liquid storage and freeze-thawing of boar semen are discussed and a review is given on examination of spermatozoa by the recent fluorescent staining methods.
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Salamons Artificial Insemination of Sheep and Goats
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Flow cytometric evaluation of sperm parameters in relation to fertility potential.
Most laboratory methods used to evaluate semen quality have not correlated highly with fertilizing capacity. The discovery of a variety of fluorochromes and compounds conjugated to fluorescent probesExpand
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Chlortetracycline analysis of boar spermatozoa after incubation, flow cytometric sorting, cooling, or cryopreservation
In this study, the effects of staining procedure with chlortetracycline (CTC) and method of analysis of boar spermatozoa after staining were examined. The hypothesis that incubation, flow cytometricExpand
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Viability and membrane integrity of spermatozoa after dilution and flow cytometric sorting in the presence or absence of seminal plasma.
Boar, bull and ram spermatozoa were examined after staining with the DNA-permeant Hoechst 33342 fluorochrome and flow cytometric sorting in the presence or absence of seminal plasma. Spermatozoa wereExpand
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Liquid storage of ram semen in the absence or presence of some antioxidants.
The antioxidants superoxide dismutase (SOD), catalase (CAT), cytochrome c (CHc) and glutathione peroxidase (GP) were added at various concentrations to Tris-glucose-yolk diluent (TGY), and theirExpand
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Seminal plasma effects on sperm handling and female fertility.
The components of ruminant seminal plasma and their influence on the fertility of spermatozoa are reviewed. Seminal plasma can both inhibit and stimulate sperm function and fertility through theExpand
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Capacitation status and fertility of fresh and frozen-thawed ram spermatozoa.
The effect of cryopreservation on the capacitation status and fertility of ram spermatozoa was observed. After the chlortetracycline staining technique was validated for ram spermatozoa, it wasExpand
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Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls.
The performance of frozen-thawed spermatozoa from 10 Holstein bulls in a range of in vitro diagnostic tests and the relationship with adjusted in vivo fertility data was determined. The testsExpand
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Effect of monosaccharides and disaccharides in Tris-based diluents on motility, acrosome integrity and fertility of pellet frozen ram spermatozoa.
Abstract In semen freezing diluents, sugars may be incorporated as metabolites, as cryoprotectants or to maintain diluent osmolality. This study examined the cryoprotective effects of mono- andExpand
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