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Food-borne trematodiases in Southeast Asia epidemiology, pathology, clinical manifestation and control.
Development of a PCR-based method for the detection of Opisthorchis viverrini in experimentally infected hamsters
In the first month of infection, the PCR method was more sensitive than using the egg-count method in all infected groups especially in the light infections and was also successfully used in monitoring a therapeutic study.
Angiostrongylus cantonensis and A. malaysiensis Broadly Overlap in Thailand, Lao PDR, Cambodia and Myanmar: A Molecular Survey of Larvae in Land Snails
This is the first report of the molecular identification of the two Angiostrongylus species in Lao PDR, Cambodia and Myanmar and the COI gene exhibited the greatest intraspecific variation in the study region.
The Opisthorchis viverrini genome provides insights into life in the bile duct
The draft genome and transcriptomes of O. viverrini are characterized and elucidate how this fluke survives in the hostile environment within the bile duct and show that metabolic pathways in the parasite are highly adapted to a lipid-rich diet from bile and/or cholangiocytes.
Fasciola gigantica-specific antigens: purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis.
- W. Maleewong, C. Wongkham, P. Intapan, V. Pipitgool
- Biology, MedicineThe American journal of tropical medicine and…
- 1 October 1999
An indirect ELISA using the antigens obtained by this relatively simple procedure was developed for detecting specific antibodies from patients infected with F. gigantica and indicated a possible correlation of antibodies to F. Gigantica with cholangiocarcinoma.
Detection of Opisthorchis viverrini in Human Stool Specimens by PCR
- S. Wongratanacheewin, W. Pumidonming, R. Sermswan, V. Pipitgool, W. Maleewong
- BiologyJournal of Clinical Microbiology
- 1 October 2002
A PCR-based method for detection of Opisthorchis viverrini in experimentally infected hamsters is successfully developed and was more sensitive than the previous method using a DNA probe as a diagnostic tool, with no amplification occurring when samples contained small intestinal flukes or other parasitic eggs.
Immunodiagnosis of human fascioliasis using an antigen of Fasciola gigantica adult worm with the molecular mass of 27 kDa by a dot-ELISA.
- P. Intapan, W. Maleewong, S. Sangmaneedet
- Biology, MedicineThe Southeast Asian journal of tropical medicine…
- 1 December 2003
A dot-ELISA using the FG 27 antigen was developed for the detection of specific antibodies from patients infected with F. gigantica and is a specific, sensitive and easy to perform method for the rapid diagnosis of fascioliasis, particularly when more complex laboratory tests are unavailable.
Molecular detection and treatment of tick-borne pathogens in domestic dogs in Khon Kaen, northeastern Thailand.
- Porntip Laummaunwai, P. Sriraj, W. Maleewong
- Medicine, BiologyThe Southeast Asian journal of tropical medicine…
- 1 September 2014
The present study suggests that the PCR technique has high sensitivity and specificity for Babesia and Ehrlichia diagnosis as well as for detection of BabesIA spp, E. canis and H.Canis DNA in EDTA blood specimens.
MOLECULAR EVIDENCE OF SPIROMETRA ERINACEIEUROPAEI INFECTION IN SNAKES PTYAS KORROS FROM LAO PDR AND THAILAND AND FROGS HOPLOBATRACHUS RUGULOSUS FROM MYANMAR.
- J. Jongthawin, P. Intapan, W. Maleewong
- BiologyThe Southeast Asian journal of tropical medicine…
- 1 November 2014
Phylogenetic analysis indicated that all spargana specimens were S. erinaceieuropaei in P. korros from Lao PDR and Thailand and H. rugulosus from Myanmar, emphasizing the need for prevention and control of sparganosis in these regions.
First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar
This study demonstrated, for the first time, O. viverrini from rural people in Myanmar using molecular methods and is an urgent call for surveillance and control activities against opisthorchiasis in Myanmar.