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Biomimetic modeling of oxidative drug metabolism
This review summarizes three different nonenzymatic strategies, including metalloporphyrins as surrogates of the active centre of cytochrome P450, Fenton’s reagent, and the electrochemical oxidation of drug compounds.
Generation and identification of reactive metabolites by electrochemistry and immobilized enzymes coupled on-line to liquid chromatography/mass spectrometry.
Electrochemical reactor and horseradish peroxidase immobilized on magnetic microparticles with liquid chromatography/mass spectrometry allows the direct detection of reactive metabolites of the model compounds amodiaquine, amsacrine, and mitoxantrone and are promising tools for the identification of both reactive and stable metabolites in drug development.
Metabolic studies of tetrazepam based on electrochemical simulation in comparison to in vivo and in vitro methods.
Electrochemistry meets enzymes: instrumental on-line simulation of oxidative and conjugative metabolism reactions of toremifene
In the absence of GST, not all GSH adducts are formed, proving the necessity of a phase II enzyme to simulate the complete metabolic pathway of xenobiotics in an on-line EC/LC/MS system.
A microfluidic chip for electrochemical conversions in drug metabolism studies.
An electrochemical cell on-chip is fabricated which is used successfully in EC-UV/vis and EC-LC-MS experiments and compared with measurements obtained with commercially available electrochemical flow-through cells.
A ferrocene-based reagent for the conjugation and quantification of reactive metabolites
- Sandra Jahn, W. Lohmann, Susanne Bomke, A. Baumann, U. Karst
- ChemistryAnalytical and Bioanalytical Chemistry
By proving the successful adduct formation between the reactive metabolite and ferrocene-labeled GSH, it could be shown that FP-GSH is an effective trapping agent which eases routine reversed-phase LC analyses.
Electrochemistry-Mass Spectrometry Unveils the Formation of Reactive Triclocarban Metabolites
Novel reactive metabolites potentially formed during biotransformation of Triclocarban are reported, and a so-far unknown dechlorinated and hydroxylated TCC metabolite has been identified.
On-line electrochemistry/liquid chromatography/mass spectrometry for the simulation of pesticide metabolism
Simulation of the detoxification of paracetamol using on-line electrochemistry/liquid chromatography/mass spectrometry
On-line electrochemistry/liquid chromatography/mass spectrometry was used to simulate the detoxification mechanism of paracetamol in the body to characterize isomeric adducts similar to those occurring between par acetamol and glutathione under catalysis by cytochrome P450 enzymes in theBody.
Covalent protein modification by reactive drug metabolites using online electrochemistry/liquid chromatography/mass spectrometry.
Due to its simple setup, easy handling, and short analysis times, the method provides an interesting tool for the rapid risk assessment of covalent protein binding as well as for the synthesis ofcovalent drug-protein adducts in high purity and high yield.