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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
TLDR
21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
RNA interference is mediated by 21- and 22-nucleotide RNAs.
TLDR
It is demonstrated that 21- and 22-nt RNA fragments are the sequence-specific mediators of RNAi, and evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the siRNA-protein complex is provided.
Identification of Novel Genes Coding for Small Expressed RNAs
TLDR
It is shown that many 21- and 22-nt expressed RNAs, termed microRNAs, exist in invertebrates and vertebrates and that some of these novel RNAs are highly conserved, which suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by smallRNAs are more general than previously appreciated.
Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate
TLDR
Duplexes of 21–23 nucleotide RNAs are the sequence‐specific mediators of RNA interference and post‐transcriptional gene silencing and mismatches in the centre of the siRNA duplex prevent target RNA cleavage, providing a rational basis for the design of siRNAs in future gene targeting experiments.
Identification of Tissue-Specific MicroRNAs from Mouse
TLDR
34 novel miRNAs were identified by tissue-specific cloning of approximately 21-nucleotide RNAs from mouse and a miRNA was identified that appears to be the fruitfly and mammalian ortholog of C. elegans lin-4 stRNA.
Small molecules that mediate RNA interference rna.
Double stranded RNA molecule isolated, wherein each RNA strand has a length from 19-25 nucleotides, wherein each RNA molecule is capable of specific nucleic acid modifications as to the target.
Small RNA molecules that mediate RNA interference.
Double stranded RNA molecule isolated, wherein each RNA strand has a length from 19-25 nucleotides, wherein each RNA molecule is capable of specific nucleic acid modifications as to the target.