The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins.
A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Isolation of a recombinant copy of the gene encoding C/EBP.
The isolation of a recombinant clone of the gene that encodes C/EBP yields a protein that binds in vitro to both the CCAAT homology and the enhancer core homology, providing conclusive evidence that a single gene product accounts for both binding activities.
The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite.
Experimental tests now provide support for the leucine zipper hypothesis, which predicts a new structural motif that plays a role in facilitating sequence-specific interaction between protein and DNA.
Differentiation-induced gene expression in 3T3-L1 preadipocytes: CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes.
Several lines of evidence indicate that the differentiation-induced nuclear factor is CCAAT/enhancer binding protein (C/EBP), a DNA-binding protein first isolated from rat liver.
Identification of a rat liver nuclear protein that binds to the enhancer core element of three animal viruses.
The identification and purification of a nuclear protein from rat liver that binds selectively to DNA sequences associated with several animal virus enhancers is described, indicating that the initial interaction established between the 20-kD protein and each viral enhancer occurs via a common DNA sequence known as the enhancer core homology.
CCAAT/enhancer binding protein activates the promoter of the serum albumin gene in cultured hepatoma cells.
It is speculated that C/EBP may play a general role in establishing and maintaining the differentiated, nonproliferative state.
Tissue-specific expression, developmental regulation, and genetic mapping of the gene encoding CCAAT/enhancer binding protein.
Observations raise the possibility that the synthesis of C/EBP may be responsive to humoral factors and that modulation in C/ EBP expression might mediate coordinated changes in gene expression that facilitate adaptive challenges met during development or during the fluctuating physiological states of adult life.
In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
We have used a recombinant bacteriophage that expresses the DNA-binding domain of C/EBP to optimize conditions for a screening technique that may facilitate the cloning of genes that encode…
Efficacy of inhaled human insulin in type 1 diabetes mellitus: a randomised proof-of-concept study
Monocarboxylate transporter expression in mouse brain.
- E. Koehler-Stec, I. Simpson, S. Vannucci, K. Landschulz, W. Landschulz
- BiologyAmerican Journal of Physiology. Endocrinology and…
- 1 September 1998
Results suggest a differential expression of the two MCTs at the cellular level, with higher levels of hybridization in hippocampus and cerebellum and lower levels in lung, brain, and epididymal fat.