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Structure and function of bacterial cold shock proteins
TLDR
The role of Csps in protein expression during cold shock with special emphasis on structural aspects is described, including the Y-box proteins that are known to be involved in regulation of several transcription and translation processes. Expand
The dynamic range of the human metabolome revealed by challenges
TLDR
It is shown that physiological challenges increased interindividual variation even in phenotypically similar volunteers, revealing metabotypes not observable in baseline metabolite profiles; volunteer‐specific metabolite concentrations were consistently reflected in various biofluids; and readouts from a systematic model of β‐oxidation showed significant and stronger associations with physiological parameters than absolute metabolite concentration. Expand
Automated assignment of NOESY NMR spectra using a knowledge based method (KNOWNOE)
TLDR
The program KNOWNOE is devised to work directly with the experimental spectra without interference of an expert and contains as a central part a knowledge driven Bayesian algorithm for solving ambiguities in the NOE assignments. Expand
Fundamental link between folding states and functional states of proteins.
TLDR
The supposed fundamental link using the Ras protein complexed with the GTP analogue GppNHp that occurs in two structural states coexisting in solution is demonstrated that state 1 represents the conformation interacting with guanine nucleotide exchange factors (GEFs). Expand
Formation of Fenestrae in Murine Liver Sinusoids Depends on Plasmalemma Vesicle-Associated Protein and Is Required for Lipoprotein Passage
TLDR
It is reported that mice, which are deficient in plasmalemma vesicle-associated protein (PLVAP), develop a distinct phenotype that is caused by the lack of sinusoidal fenestrations, which substantially impairs the passage of chylomicron remnants between liver sinusoids and hepatocytes, and finally leads to liver damage. Expand
Backbone dynamics of sequence specific recognition and binding by the yeast Pho4 bHLH domain probed by NMR
TLDR
It is shown that formation of either the nonspecific and cognate DNA bound complexes involves a large change in conformation and backbone dynamics of the basic region, and evidence for conformational flexibility at a highly conserved glutamate basic region residue is presented. Expand
Solution NMR structure of the cold-shock protein from the hyperthermophilic bacterium Thermotoga maritima.
TLDR
The side chain of R2 probably participates in a peripheral ion cluster involving residues D20, R2, E47 and K63, suggesting that the thermostability of TmCsp is based on the peripheral ion clusters around the side chainof R2. Expand
Observation of intermediate states of the human prion protein by high pressure NMR spectroscopy
TLDR
High-pressure NMR spectroscopy indicates that the folded core of the human prion protein occurs in two structural states N1and N2 in solution associated with rather small differences in free enthalpies (3.0 kJ/mol). Expand
Structure of the leech protein saratin and characterization of its binding to collagen.
TLDR
The three-dimensional structure of the leech protein Saratin in solution is solved and its collagen binding site is identified by NMR titration experiments, revealing a kind of notch that is formed by the end of the alpha-helix and the beta-sheet. Expand
Structure determination and ligand interactions of the PDZ2b domain of PTP-Bas (hPTP1E): splicing-induced modulation of ligand specificity.
TLDR
The solution structure of the splicing variant of PDZ2 with the insertion has been determined using 2D and 3D heteronuclear NMR experiments and the structural reason for the changed binding specificity is the reorientation of the loop with extra five amino acid residues, which folds back onto beta-strands two and three. Expand
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