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Genetic variability of apple proliferation phytoplasmas as determined by PCR-RFLP and sequencing of a non-ribosomal fragment.
Apple proliferation phytoplasmas are considered as quarantine organisms in Europe and north America, but reliable polymerase chain reaction (PCR) primers for their identification in routine diagnosisExpand
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Detection and Indentification of European Stone Fruit Yellows and Other Phytoplasmas in Wild Plants in the Surroundings of Apricot Chlorotic Leaf Roll-affected Orchards in Southern France
Between 1994 and 1998 a field study was conducted to identify plant hosts of the European stone fruit yellows (ESFY) phytoplasma in two apricot growing regions in southern and southwestern FranceExpand
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Tetanus toxin: primary structure, expression in E. coli, and homology with botulinum toxins.
A pool of synthetic oligonucleotides was used to identify the gene encoding tetanus toxin on a 75‐kbp plasmid from a toxigenic non‐sporulating strain of Clostridium tetani. The nucleotide sequenceExpand
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Differentiation of mycoplasmalike organisms (MLOs) in European fruit trees by PCR using specific primers derived from the sequence of a chromosomal fragment of the apple proliferation MLO.
A 1.8-kb chromosomal DNA fragment of the mycoplasmalike organism (MLO) associated with apple proliferation was sequenced. Three putative open reading frames were observed on this fragment. TheExpand
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Multilocus sequence analysis reveals the genetic diversity of European fruit tree phytoplasmas and supports the existence of inter-species recombination.
The genetic diversity of three temperate fruit tree phytoplasmas 'Candidatus Phytoplasma prunorum', 'Ca. P. mali' and 'Ca. P. pyri' has been established by multilocus sequence analysis. Among theExpand
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Pcr Assay for specific detection of European stone fruit yellows phytoplasmas and its use for epidemiological studies in France
DNA amplification by polymerase chain reaction was used to specifically detect phytoplasmas associated with severe decline diseases of European stone fruits. PCR primers were designed according toExpand
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Delivery of Hairpin RNAs and Small RNAs Into Woody and Herbaceous Plants by Trunk Injection and Petiole Absorption
Since its discovery, RNA interference has been widely used in crop protection. Recently, transgene-free procedures that were based on exogenous application of RNA molecules having the capacity toExpand
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Effect of recombinant beet necrotic yellow vein virus with different RNA compositions on mechanically inoculated sugarbeets.
Beet necrotic yellow vein virus (BNYVV) inocula with different RNA compositions were prepared from infectious transcripts of RNAs 3 and 4 and the Rg 1 isolate, which has a genome consisting only ofExpand
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Cacopsylla melanoneura has no relevance as vector of apple proliferation in Germany.
Long-term field surveys on the distribution and natural infection rates of Cacopsylla melanoneura were carried out in commercial and abandoned apple-proliferation-infected orchards throughoutExpand
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