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Electron cryo-microscopy shows how strong binding of myosin to actin releases nucleotide
By fitting atomic models of actin and the myosin cross-bridge into high-resolution electron cryo-microscopy three-dimensional reconstructions, the molecular basis of this linkage is described and the closing of the actin-binding cleft when actin binds is structurally coupled to the opening of the nucleotide-binding pocket.
Argiotoxin detects molecular differences in AMPA receptor channels
Network formation of lipid membranes: triggering structural transitions by chain melting.
- M. F. Schneider, D. Marsh, W. Jahn, B. Kloesgen, T. Heimburg
- ChemistryProceedings of the National Academy of Sciences…
- 7 December 1999
Cryo-transmission and freeze-fracture electron microscopy are combined here with calorimetry and viscometry to demonstrate the reversible conversion of phosphatidylglycerol aqueous vesicle suspensions to a three-dimensional structure that consists of extended bilayer networks.
Microstructure of microemulsions by freeze fracture electron microscopy
Refroidissement rapide de microemulsions dans le propane liquide. La fracture sous vide et la replique d'une face fracturee permet la discrimination des domaines riches en eau et huile par…
Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative.
The structural data obtained when combined with various biochemical constraints enabled us to critically evaluate two distinct atomic models of the F-actin filament (i.e. the Holmes-Lorenz versus the Schutt-Lindberg model).
Three-dimensional atomic model of F-actin decorated with Dictyostelium myosin S1
An atomic model for the actomyosin complex is produced by combining the atomic X-ray structure of F-actin3,4 and chicken myosin S15 with a three-dimensional reconstruction from electron micrographs of frozen-hydrated F- actin decorated with recombinant Dictyostelium myOSin S1.
Reversal of Interlaminar Signal Between Sensory and Memory Processing in Monkey Temporal Cortex
Methods of preparing well-orientated sols of f-actin containing filaments suitable for X-ray diffraction.
Freeze fracture electron microscopy of dilute lamellar and anomalous isotropic (L3) phases
We show images of dilute lamellar (Lα) and anomalolus isotropic (L 3 ) phases obtained by freeze fracture electron microscopy. The images of the Lα phase show stacks of bilayers. In some parts,…