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The nutrition of Paramecium aurelia, stock 299.
SYNOPSIS. Paramecium aurelia, stock 299 (symbiote-free) was cultivated in a synthetic medium consisting of amino acids, vitamins, purine and pyrimidine derivatives, fatty acids, stigmasterol, sodium…
Nucleic acid and protein content of purified endosymbiote particles of Paramecium aurelia.
The surface antigens of Paramecium aurelia.
- R. Litman, A. Reisner, B. VAN TIJN, W. J. van Wagtendonk, M. Young
- BiologyJournal of general microbiology
- 1 December 1956
SUMMARY: A surface antigen preparation obtained from Paramecium aurelia absorbs from a concentrated antiserum approximately 74% of the total antibody absorbed by the intact animal. An antiserum…
The isolation from lemon juice of a growth factor of steroid nature required for the growth of a strain of Paramecium aurelia.
SUMMARY: A steroid fraction obtained from lemon juice was found to be an essential metabolite for Paramecium aurelia, var. 4, stock 51.7 (s), in axenic culture. This steroid is closely related to, or…
Steroid requirements of Paramecium aurelia.
The specificity of these requirements suggests that the steroid functions as an essential metabolite for this organism.
An analysis of the nutritional requirements for fatty acids of Paramecium aurelia.
Tween 60, a non-ionic emulsifier similar in its surfactant properties to Tween 80, stimulated growth in the presence of suboptimal amounts of oleic acid but failed itself to replace oleoic acid as a growth requirement.
GROWTH REQUIREMENTS OF PARAMECIUM AURELIA VAR. 4, STOCK 51.7 SENSITIVES AND KILLERS IN AXENIC MEDIUM
- W. J. van Wagtendonk, R. Conner, C. Miller, M. Rao
- BiologyAnnals of the New York Academy of Sciences
- 1 October 1953
The nutritional requirements of only a few ciliated protozoa have been investigated successfully, especially those relating to the single genus Tetrahymena, and it was found that P. aurelia can be grown in an axenic medium consisting of heat-stable components.
The Culture of Paramecium Aurelia in the Absence of Other Living Organisms.
- W. J. van Wagtendonk, P. Hackett
- MathematicsProceedings of the National Academy of Sciences…
- 1 March 1949
incubated three tem-peratures,
Evidence for the presence of a protein with ATP-ase and antigenic specificity in Paramecium aurelia, variety 4, stock 51.
A method for the mass collection of axenically cultivated Paramecium aurelia.
The procedure takes advantage of the fact that the ciliates are negatively geotropic and involves migrating the organisms directly from the culture medium into an overlayer of salt solution of low density.