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The nutrition of Paramecium aurelia, stock 299.
SYNOPSIS. Paramecium aurelia, stock 299 (symbiote-free) was cultivated in a synthetic medium consisting of amino acids, vitamins, purine and pyrimidine derivatives, fatty acids, stigmasterol, sodiumExpand
Nucleic acid and protein content of purified endosymbiote particles of Paramecium aurelia.
TLDR
A new method has been developed for the isolation and purification of endosymbiote particles λ, μ, and π from axenically cultivated Paramecium aurelia based on differential centrifugation of homogenates containing the particles in NaBr solution of different densities. Expand
The surface antigens of Paramecium aurelia.
SUMMARY: A surface antigen preparation obtained from Paramecium aurelia absorbs from a concentrated antiserum approximately 74% of the total antibody absorbed by the intact animal. An antiserumExpand
Steroid requirements of Paramecium aurelia.
TLDR
The specificity of these requirements suggests that the steroid functions as an essential metabolite for this organism. Expand
An analysis of the nutritional requirements for fatty acids of Paramecium aurelia.
TLDR
Tween 60, a non-ionic emulsifier similar in its surfactant properties to Tween 80, stimulated growth in the presence of suboptimal amounts of oleic acid but failed itself to replace oleoic acid as a growth requirement. Expand
GROWTH REQUIREMENTS OF PARAMECIUM AURELIA VAR. 4, STOCK 51.7 SENSITIVES AND KILLERS IN AXENIC MEDIUM
TLDR
The nutritional requirements of only a few ciliated protozoa have been investigated successfully, especially those relating to the single genus Tetrahymena, and it was found that P. aurelia can be grown in an axenic medium consisting of heat-stable components. Expand
The Culture of Paramecium Aurelia in the Absence of Other Living Organisms.
Evidence for the presence of a protein with ATP-ase and antigenic specificity in Paramecium aurelia, variety 4, stock 51.
TLDR
A protein with adenosinetriphosphate activity has been isolated from P. aurelia and gives a precipitate with an antiserum prepared against serotype 51A. Expand
The metabolism of stigmasterol and cholesterol by Paramecium aurelia.
TLDR
The data indicate that a correlation between molecular structure and biological activity may reside in the nature of the substituent at C-24, and the physiological specificity can be envisioned as a structural membrane requirement or in terms of a sterol capable of serving as a substrate for fatty acid esterification. Expand
A method for the mass collection of axenically cultivated Paramecium aurelia.
TLDR
The procedure takes advantage of the fact that the ciliates are negatively geotropic and involves migrating the organisms directly from the culture medium into an overlayer of salt solution of low density. Expand
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