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Crystal structure and site-directed mutagenesis studies of N-carbamoyl-D-amino-acid amidohydrolase from Agrobacterium radiobacter reveals a homotetramer and insight into a catalytic cleft.
The N-carbamoyl-D-amino-acid amidohydrolase (D-NCAase) is used on an industrial scale for the production of D-amino acids. The crystal structure of D-NCAase was solved by multiple isomorphousExpand
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Production and properties of a raw‐starch‐degrading amylase from the thermophilic and alkaliphilic Bacillus sp. TS‐23
The optimum temperature and initial medium pH for amylase production by Bacillus sp. TS‐23 were 55 °C and 8.5 respectively. Maximum amylase activity was obtained in a medium containing peptone andExpand
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Penicillium and related Teleomorphs from Taiwan
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Identification of the open reading frame for the Pseudomonas putida D-hydantoinase gene and expression of the gene in Escherichia coli.
A DNA fragment containing the gene for D-hydantoinase was cloned from Pseudomonas putida CCRC 12857 into Escherichia coli. The cloned gene contained an open reading frame (ORF) of 1485 nucleotidesExpand
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Efficient utilization of starch by a recombinant strain of Saccharomyces cerevisiae producing glucoamylase and isoamylase
Two plasmids, designated pRTI and pTI, were constructed to allow the integration of a bacterial isoamylase gene (iso) into Saccharomyces cerevisiae G23‐8 chromosome. The integrative plasmid pRTIExpand
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Serine 187 is a crucial residue for allosteric regulation of Corynebacterium glutamicum 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase.
Corynebacterium glutamicum 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase is sensitive to feedback inhibition by tyrosine. One feedback-insensitive mutant was obtained by in vitroExpand
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Lactose‐induced expression of Bacillus sp. TS‐23 amylase gene in Escherichia coli regulated by a T7 promoter
Bacillus sp. TS‐23 amylase gene was amplified by polymerase chain reaction and cloned into the pET23(+) transcription vector. Lactose induction of Escherichia coli BL21(DE3) cells harbouring thisExpand
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Expression, crystallization and preliminary X-ray diffraction studies of N-carbamyl-D-amino-acid amidohydrolase from Agrobacterium radiobacter.
The Agrobacterium radiobacter CCRC 14924 N-carbamyl-D-amino-acid amidohydrolase, the enzyme used for production of D-amino acids, was overexpressed in Escherichia coli JM109. The expressed proteinExpand
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Production of isoamylase by Pseudomonas amyloderamosa mutant strain JD210.
Nutritional requirements for the production of isoamylase by Pseudomonas amyloderamosa mutant strain JD210 were investigated. The optimal initial pH for enzyme production in shake-flask cultivationExpand
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