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Zebrafish Gonadotropins and Their Receptors: I. Cloning and Characterization of Zebrafish Follicle-Stimulating Hormone and Luteinizing Hormone Receptors— Evidence for Their Distinct Functions in…
Abstract In the present study, we cloned and characterized zebrafish FSH receptor (Fshr) and LH receptor (Lhr). Both fshr and lhr were abundantly expressed in the zebrafish gonads; however, they… Expand
Zebrafish Gonadotropins and Their Receptors: II. Cloning and Characterization of Zebrafish Follicle-Stimulating Hormone and Luteinizing Hormone Subunits—Their Spatial-Temporal Expression Patterns and…
Abstract Gonadotropins, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) play critical roles in vertebrate reproduction. In the present study, we cloned and characterized zebrafish… Expand
Induction of laccase activity in the edible straw mushroom, Volvariella volvacea.
Volvariella volvacea, strain V14, produces multiple forms of extracellular laccase when grown in submerged culture in a defined medium with glucose as sole carbon source, and on cotton waste… Expand
Growth Differentiation Factor 9 and Its Spatiotemporal Expression and Regulation in the Zebrafish Ovary1
Abstract Growth differentiation factor 9 (GDF9) is a member of the transforming growth factor beta (TGFB) superfamily. As an oocyte-specific growth factor, GDF9 plays critical roles in controlling… Expand
Genetic analysis of zebrafish gonadotropin (FSH and LH) functions by TALEN-mediated gene disruption.
Vertebrate reproduction is controlled by two gonadotropins (FSH and LH) from the pituitary. Despite numerous studies on FSH and LH in fish species, their functions in reproduction still remain poorly… Expand
Developmental Profiles of Activin βA, βB, and Follistatin Expression in the Zebrafish Ovary: Evidence for Their Differential Roles During Sexual Maturation and Ovulatory Cycle1
Abstract Our recent experiments showed that gonadotropin(s) stimulated activin βA and follistatin expression through the cAMP-PKA pathway but suppressed βB via a cAMP-dependent but PKA-independent… Expand
Gonadotropin and Activin Enhance Maturational Competence of Oocytes in the Zebrafish (Danio rerio)1
Abstract In most teleosts, 17α,20β-dihydroxy-4-pregnen-3-one (DHP) serves as the most potent maturation-inducing steroid (MIS) to initiate final oocyte maturation. The maturational competence or the… Expand
Cloning of zebrafish ovarian P450c17 (CYP17, 17alpha-hydroxylase/17, 20-lyase) and characterization of its expression in gonadal and extra-gonadal tissues.
Cytochrome P450c17 (CYP17, 17alpha-hydroxylase/17, 20-lyase) has been viewed as a critical enzyme for the biosynthesis of gonadal and adrenal steroids in vertebrates. A full-length cDNA coding for… Expand
Co-metabolic biodegradation of acetamiprid by Pseudoxanthomonas sp. AAP-7 isolated from a long-term acetamiprid-polluted soil.
An AAP-degrading bacterium, AAP-7, was isolated from AAP-polluted soil. AAP-7 was identified as Pseudoxanthomonas sp. on the basis of the comparative analysis of 16S rDNA sequences. The strain was… Expand
Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea.
We have isolated a laccase (lac1) from culture fluid of Volvariella volvacea, grown in a defined medium containing 150 micro m CuSO4, by ion-exchange and gel filtration chromatography. Lac1 has a… Expand