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CTR1 phosphorylates the central regulator EIN2 to control ethylene hormone signaling from the ER membrane to the nucleus in Arabidopsis
TLDR
It is demonstrated that CTR1 interacts with and directly phosphorylates the cytosolic C-terminal domain of EIN2, an ER membrane-localized Nramp homolog that positively regulates ethylene responses, and significantly advance the understanding of the mechanisms underlying ethylene signal transduction. Expand
Comparison of protein solubilization methods suitable for proteomic analysis of soybean seed proteins.
TLDR
The results suggest that the thiourea/urea and TCA methods are efficient and reliable methods for 2D separation of soybean seed proteins and subsequent identification by mass spectrometry. Expand
Proteomic analyses of male contributions to honey bee sperm storage and mating
TLDR
Of the 69 unique honey bee proteins found, 66 are also in Drosophila melanogaster, and there is over‐representation of genes implicated in the glycolysis pathway. Expand
Protein accumulation in the germinating Uromyces appendiculatus uredospore.
TLDR
Germlings contained a higher amount of proteins involved in mitochondrial ADP:ATP translocation, which is indicative of increased energy production, and there were more accumulating histone proteins, pointing to the reorganization of the nuclei that occurs after germination prior to appressorium formation. Expand
Dual labeling of the cytoskeleton and DNA strand breaks in porcine embryos produced in vivo and in vitro
TLDR
Data suggest that the combination of these labeling techniques may be useful in evaluating cellular damage in embryos produced under in vitro conditions, and Microtubule and microfilament labeling identified blastomeres of unequal size and shape that were losing cellular integrity. Expand
An efficient extraction method to enhance analysis of low abundant proteins from soybean seed.
TLDR
The results suggest that extraction of soybean seed powder with 40% isopropanol enriches lower abundance proteins and is a suitable method for 2D-PAGE separation and identification. Expand
Bovine Blastocyst-Derived Trophectoderm and Endoderm Cell Cultures: Interferon Tau and Transferrin Expression as Respective In Vitro Markers
TLDR
This simple coculture method for the in vitro propagation of bovine trophectoderm and endoderm provides a system for assessing their biology in vitro. Expand
Characterization of storage proteins in wild (Glycine soja) and cultivated (Glycine max) soybean seeds using proteomic analysis.
TLDR
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) with three different immobilized pH gradient (IPG) strips was an effective method to separate a large number of abundant and less-abundant storage proteins. Expand
Effects of aromatase inhibitors, aminoglutethimide, and 4-hydroxyandrostenedione on cyclic rats and rats with 7,12-dimethylbenz(a)anthracene-induced mammary tumors.
TLDR
4-Hydroxyandrostenedione which inactivates aromatase may also prevent new enzyme synthesis by directly inhibiting gonadotropins and this would result in more effective reduction in ovarian estrogen production by 4-OHA than AG during long-term treatment. Expand
Transcriptome profiling of the tubular porcine conceptus identifies the differential regulation of growth and developmentally associated genes
TLDR
The current study aim was to identify genes required for elongation by defining the transcriptome profile of this dynamic tubular stage by examining the gastrulation and proliferative status of ovoid, tubular, and filamentous conceptuses. Expand
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