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New isolates and physiological properties of the Aquificales and description of Thermocrinis albus sp. nov.
TLDR
The ecology of the Aquificales was studied using a combination of phylogenetic and cultivation approaches, and a novel isolate obtained from whitish streamers in the Hveragerthi area of Iceland represents a second species within the order Thermocrinis, which is named Thermocrats albus HI 11/12. Expand
Signature Lipids and Stable Carbon Isotope Analyses of Octopus Spring Hyperthermophilic Communities Compared with Those ofAquificales Representatives
TLDR
The depletion in the C20–21 fatty acids from the PSC indicates that Thermocrinis biomass must be similarly depleted and too light to be explained by growth on CO2, while the molecular and isotopic compositions of lipid biomarkers of cultured Aquificales genera were used to study the community and trophic structure of the hyperthermophilic pink streamers and vent biofilm from Octopus Spring. Expand
Thermocrinis ruber gen. nov., sp. nov., a Pink-Filament-Forming Hyperthermophilic Bacterium Isolated from Yellowstone National Park
TLDR
A novel hyperthermophilic bacterium was isolated from pink filamentous streamers occurring in the upper outflow channel of Octopus Spring in Yellowstone National Park, Wyo, and represents a new genus in the order Aquificales, the type species of which is namedThermocrinis ruber (type strain, OC 1/4 [= DSM 12173]). Expand
Novel 16S rRNA gene sequences retrieved from highly saline brine sediments of Kebrit Deep, Red Sea
TLDR
The results suggest that hitherto unknown archaea and bacteria may thrive in highly saline brines of the Red Sea under extreme environmental conditions. Expand
A Reliable Tool to Determine Cell Viability in Complex 3-D Culture: The Acid Phosphatase Assay
TLDR
A panel of standard assays were explored to provide an easy-handling, standardized protocol for rapid routine analysis of cell survival in multicellular tumor spheroid-based antitumor drug testing, and one of the assay systems tested could reliably be used to determine cell viability in spheroids. Expand
Microbial Diversity of the Brine-Seawater Interface of the Kebrit Deep, Red Sea, Studied via 16S rRNA Gene Sequences and Cultivation Methods
TLDR
It is demonstrated that novel halophiles thrive in the anaerobic, deep-sea brine pool of the Kebrit Deep, Red Sea, and may contribute significantly to theAnaerobic degradation of organic matter enriched at the brine-seawater interface. Expand
Prokaryotic phylogenetic diversity and corresponding geochemical data of the brine-seawater interface of the Shaban Deep, Red Sea.
TLDR
The interface between the hypersaline brine and the overlying sea-water (brine-seawater interface) of the Shaban Deep, northern Red Sea was investigated for the presence of microorganisms using the 16S rRNA gene as a molecular marker, and novel representatives of the KB1 sequence group were detected indicating a widespread distribution of the corresponding Bacteria in Deep Sea brine pools. Expand
Salinisphaerashabanensis gen. nov., sp. nov., a novel, moderately halophilic bacterium from the brine–seawater interface of the Shaban Deep, Red Sea
TLDR
The creation of the new genus Saliniphaera and the new species Salinisphaera shabanensis is proposed for this new and highly versatile microorganism. Expand
Description of Undibacterium oligocarboniphilum sp. nov., isolated from purified water, and Undibacterium pigrum strain CCUG 49012 as the type strain of Undibacterium parvum sp. nov., and emended
TLDR
16S rRNA gene sequence similarity values lower than 97.0 % and several differentiating phenotypic traits demonstrated that strain EM 1(T) represents a novel species for which the name Undibacterium oligocarboniphilum sp. Expand
Hydrobacter penzbergensis gen. nov., sp. nov., isolated from purified water.
TLDR
16S rRNA gene sequence analysis and the polar lipid and fatty acid profiles suggest that the strain represents a novel genus and species, for which the name Hydrobacter penzbergensis gen. nov., sp. Expand
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