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Structure of human estrogenic 17 beta-hydroxysteroid dehydrogenase at 2.20 A resolution.
The structure of the active site provides a rational basis for designing more specific inhibitors of this breast cancer associated enzyme and a model for steroid and cofactor binding as well as for the estrone to estradiol transition state is proposed. Expand
The refined three-dimensional structure of 3α,20β-hydroxysteroid dehydrogenase and possible roles of the residues conserved in short-chain dehydrogenases
Abstract Background Bacterial 3 α ,20 β -hydroxysteroid dehydrogenase reversibly oxidizes the 3 α and 20 β hydroxyl groups of steroids derived from androstanes and pregnanes. It was the first… Expand
Porcine Carbonyl Reductase
- D. Ghosh, M. Sawicki, +4 authors W. Duax
- Chemistry, Medicine
- The Journal of Biological Chemistry
- 25 May 2001
A 41-residue insertion immediately before the catalytic Tyr describes an all-helix subdomain that packs against interfacial helices, eliminating the four-helIX bundle interface conserved in the SDR superfamily. Expand
Structure of uncomplexed and linoleate-bound Candida cylindracea cholesterol esterase.
The present study explores the details of dimer formation of CE and the basis for its substrate specificity, revealing a dimeric association of monomers in which two active-site gorges face each other, shielding hydrophobic surfaces from the aqueous environment. Expand
Structure/Function Relationships Responsible for Coenzyme Specificity and the Isomerase Activity of Human Type 1 3β-Hydroxysteroid Dehydrogenase/Isomerase*
- James L. Thomas, W. Duax, A. Addlagatta, Stacey Brandt, R. R. Fuller, W. Norris
- Biology, Medicine
- Journal of Biological Chemistry
- 12 September 2003
Kinetic analyses and pH dependence studies showed that either Asp257 or Asp258 plays a catalytic role in the isomerization reaction, bringing the goal of selectively inhibiting the type 1 enzyme in placenta or in hormone-sensitive breast tumors closer. Expand
Steroid dehydrogenase structures, mechanism of action, and disease.
The three-dimensional structure of the 3 alpha, 20 beta-HSD carbenoxolone complex unequivocally verifies the postulated active site of the enzyme, shows that inhibition is a result of direct competition with the substrate for binding, and provides a plausible model for the mechanism of inhibition of 11 beta-hydroxysteroid dehydrogenase by carben oxolone. Expand
Mechanism of inhibition of 3α,20β-hydroxysteroid dehydrogenaseby a licorice-derived steroidal inhibitor
Abstract Background: Bacterial 3 α , 20 β -hydroxysteroid dehydrogenase (3 α , 20 β -HSD) reversibly oxidizes the 3 α and 20 β hydroxyl groups of androstanes and pregnanes and uses nicotinamide… Expand
Did tRNA synthetase classes arise on opposite strands of the same gene?
Structural homology of class II aminoacyl-tRNA synthetases to the HSP70 family and the existence of a gene whose sense and antisense strands code for a dehydrogenase and an HSP70 chaperonin justify… Expand