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Stimulus‐secretion coupling: the concept and clues from chromaffin and other cells
  • W. Douglas
  • Biology, Medicine
  • British journal of pharmacology
  • 1 November 1968
This lecture summarizes the knowledge in a field dubbed autopharmacology: the secretion by a body cell of a substance prompted by an internal stimulus with the adrenal chromaffin cell and focuses on calcium and stumulus secretion coupling in other systems including the exocrine glands neurones and neurosecretory cells. Expand
The role of calcium in the secretory response of the adrenal medulla to acetylcholine
The experiments show that the excitant action of ACh on the adrenal medulla is dependent on the presence of calcium, and suggest that ACh evokes adrenalmedullary secretion by causing calcium ions to penetrate the adrenAL medullary cells. Expand
Calcium-induced extrusion of secretory granules (exocytosis) in mast cells exposed to 48-80 or the ionophores A-23187 and X-537A.
Support is considered for the view that Ca influx mediates stimulus-secretion coupling and does so by initiating exocytosis, and responses seem independent of possible shifts in the alkali metal ions. Expand
Non‐medullated fibres in the saphenous nerve which signal touch
Catecholamines of supposed inhibitory hypophysiotrophic function suppress action potentials in prolactin cells
It is reported that normal prolactin cells generate spontaneous action potentials which are suppressed by hypothalmic catecholamines known to inhibit Prolactin secretion, and the evidence which supports this scheme is scanty. Expand
Depressor reflexes from medullated and non‐medullated fibres in the rabbit's aortic nerve
Two distinct groups of afferents are revealed in the aortic nerve of the rabbit, both of which reflexly lower the blood pressure and which appear to be non-medullated, and whose reflex depressor effects are long-lasting and powerful. Expand
The excitant action of acetylcholine and other substances on cutaneous sensory pathways and its prevention by hexamethonium and d‐tubocurarine
The experiments described in this paper show that a-lobeline, like acetylcholine and nicotine, can excite a discharge in sensory fibres from the skin; and that hexamethonium and D-tubocurarine prevent the excitant effect of this group of drugs without influencing the normal touch response. Expand
Effects of acetylcholine and other medullary secretagogues and antagonists on the membrane potential of adrenal chromaffin cells: an analysis employing techniques of tissue culture
1. A method has been devised for isolating adrenal chromaffin cells (from gerbils) and maintaining them in vitro. Transmembrane potentials of these cells were recorded with intracellularExpand