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Structural features of the binding site of cholera toxin inferred from fluorescence measurements.
The dependence on pH of the fluorescence of cholera toxin and its A and B subunits has been studied at 25 degrees C. The fluorescence intensity of cholera toxin is highly pH-dependent. In the pHExpand
Nucleotide sequence analysis of the CT operon of the Vibrio cholerae classical strain 569B.
The complete nucleotide sequence of the Vibrio cholerae classical strain 569B was determined. The results prove the exactness of the amino acid CT B sequence published by Takao et al. (1985, Eur. J.Expand
Interaction of a cholera toxin derivative containing a reduced number of receptor binding sites with intact cells in culture.
Hybrid CTB (hCTB), having only one or two functional binding sites, has been constructed from two chemically inactivated derivatives of CTB. One inactive derivative consisted of CTB formylated in theExpand
Subcellular structure of bovine thyroid gland. A study on bovine thyroid membranes by buoyant-density-gradient centrifugation in a B-XIV zonal rotor.
A combined mitochondrial and light mitochondrial fraction and a microsomal fraction were isolated from bovine thyroid gland and fractionated further in a B-XIV zonal rotor. A density gradient rangingExpand
Phospholipases A1 and A2 in bovine thyroid.
In both supernatant and sediment of thyroid tissue homogenate phospholipase and lysophospholipase activities were demonstrated. In the supernatant, usingExpand
The nuclear lipids of bovine hypertrophic thyroid.
Abstract A highly purified preparation of bovine hypertrophie thyroid nuclei was isolated by means of an adapted method of Widnell, C. C. and Tata, J. R. (1964) Biochem. J. 92, 313–317, followed by aExpand
Vascular and noradrenalic reactions in the musculocutaneous bed during hypothalamic stimulation.
When electrical stimulation is applied to the ventromedial hypothalamic zone one observes an increase in systemic blood pressure. There also occur blood pressure variations in the isolated femoralExpand
Multiple system atrophies. A neuropathological and neurochemical study.
Abstract The 5 familial cases which are reported here, showed a similar clinical course characterized by an early onset and a very long duration provided adequate nursing care was available. The mainExpand
Catecholamines in blood and myocardial tissue in experimental subarachnoidal hemorrhage.
The induction of experimental subarachnoidal hemorrhage (ESH) in the dog results in a rise in the adrenaline plasma concentration. The changes in plasma noradrenaline are biphasic: an ‘early noradrena
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