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Improvement of artemisinin production by chitosan in hairy root cultures of Artemisia annua L.
- W. Putalun, Wanwimon Luealon, W. De-Eknamkul, H. Tanaka, Y. Shoyama
- Biology, Medicine
- Biotechnology Letters
- 11 April 2007
Artemisinin production by hairy roots of Artemisia annua L. was increased 6-fold to 1.8 μg mg−1 dry wt over 6 days by adding 150 mg chitosan l−1. The increase was dose-dependent. Similar treatment of… Expand
Biosynthesis of beta-sitosterol and stigmasterol in Croton sublyratus proceeds via a mixed origin of isoprene units.
A green callus culture of Croton sublyratus Kurz established from the leaf explants appeared to actively synthesize two well-known phytosterols, beta-sitosterol and stigmasterol. The phytosterol… Expand
Tyrosine aminotransferase: The entrypoint enzyme of the tyrosine-derived pathway in rosmarinic acid biosynthesis
Abstract High activity of tyrosine aminotransferase (TAT; EC 184.108.40.206) was found in extracts of rosmarinic acid-producing cell cultures of Anchusa officinalis and Coleus blumei , while low activity of… Expand
Aromatic and pyrone polyketides synthesized by a stilbene synthase from Rheum tataricum.
- S. Samappito, J. Page, J. Schmidt, W. De-Eknamkul, T. Kutchan
- Biology, Medicine
- 1 February 2003
A cDNA encoding a stilbene synthase, RtSTS, was isolated from the rhizomes of Tatar rhubarb, Rheum tataricum L. (Polygonaceae), a medicinal plant containing stilbenes and other polyketides.… Expand
Purification and characterization of tyrosine aminotransferase activities from Anchusa officinalis cell cultures.
Three activities of tyrosine aminotransferase (TAT; EC 220.127.116.11), the enzyme which catalyzes the first step of the tyrosine pathway leading to the formation of rosmarinic acid… Expand
Potential targets in the discovery of new hair growth promoters for androgenic alopecia
Introduction: Androgenic alopecia (AGA) is the major type of scalp hair loss affecting 60 – 70% of the population worldwide. It is caused by two potent androgens, namely testosterone (T) and… Expand
Geranylgeraniol-18-hydroxylase: the last enzyme on the plaunotol biosynthetic pathway in Croton sublyratus
Abstract The activity of geranylgeraniol-18-hydroxylase, a novel enzyme catalysing the C-18 hydroxylation of geranylgeraniol (GGOH) to plaunotol, has been demonstrated in a cell-free extract prepared… Expand
Effects of macronutrients on growth and rosmarinic acid formation in cell suspension cultures of Anchusa officinalis
The influence of various macronutrients on growth and RA formation in cell suspension cultures of Anchusa officinalis has been investigated. Factors tested included sucrose concentration, alternate… Expand
Monitoring of inflammatory mediators induced by silk sericin.
- P. Aramwit, S. Kanokpanont, W. De-Eknamkul, T. Srichana
- Chemistry, Medicine
- Journal of bioscience and bioengineering
- 1 May 2009
Silk proteins have been shown to be good candidates for biomedical materials. However, there have been some reports regarding immunological and allergic responses to silk sericin. Our objective was… Expand
The Effect of Sericin with Variable Amino-Acid Content from Different Silk Strains on the Production of Collagen and Nitric Oxide
- P. Aramwit, S. Kanokpanont, W. De-Eknamkul, K. Kamei, T. Srichana
- Materials Science, Medicine
- Journal of biomaterials science. Polymer edition
- 1 January 2009
Although silk sericin (SS) enhances the growth and attachment of fibroblast cells, its toxicity remains questionable. We investigated the effect of SS extracted by heat with variable amino-acid… Expand