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Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome.
TLDR
A sophisticated viral counter-defense strategy that targets the silencing antiviral pathway at multiple steps is revealed and may be essential for protecting CTV with such a large RNA genome from antiviral silencing in the perennial tree host.
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Heterologous sequences greatly affect foreign gene expression in tobacco mosaic virus-based vectors.
TLDR
There was an inverse relationship between expression of GFP and the heterologous coat protein genes that was reflected in accumulation of the respective mRNAs and proteins, and the most effective vector contained sequences encoding the coat protein subgenomic mRNA promoter, coat protein ORF, and 3' nontranslated region from tobacco mild green mosaic virus U5.
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Sequences of Citrus Tristeza VirusSeparated in Time and Space Are Essentially Identical
ABSTRACT The first Citrus tristeza virus (CTV) genomes completely sequenced (19.3-kb positive-sense RNA), from four biologically distinct isolates, are unexpectedly divergent in nucleotide sequence…
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Examination of the responses of different genotypes of citrus to huanglongbing (citrus greening) under different conditions.
TLDR
Examination of responses of 30 different genotypes of citrus to Florida isolates of 'Ca. L. asiaticus' provides additional evidence of the correlation between disruption of phloem translocation of carbohydrates during HLB infection and the appearance of chlorotic symptoms in leaves of infected trees.
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Systemic expression of a bacterial gene by a tobacco mosaic virus-based vector.
TLDR
A hybrid viral RNA (TB2) was constructed, containing sequences from two tobamoviruses (TMV-U1 and odontoglossum ringspot virus), providing a rapid means of expressing genes and gene variants in plants.
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Closterovirus bipolar virion: evidence for initiation of assembly by minor coat protein and its restriction to the genomic RNA 5' region.
The long flexuous virions of the Closteroviridae have a unique bipolar architecture incorporating two coat proteins, with most of the helical nucleocapsid encapsidated by the major coat protein (CP)…
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The complete genome sequence of the major component of a mild citrus tristeza virus isolate.
TLDR
The genome of the Spanish mild isolate T385 of citrus tristeza virus was completely sequenced and compared with the genomes of the severe isolates T36, VT and SY568, suggesting that the central region in the SY568 genome results from RNA recombination between two CTV genomes, one of which was almost identical to T385.
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Mapping of the Tobacco mosaic virus movement protein and coat protein subgenomic RNA promoters in vivo.
TLDR
The Tobacco mosaic virus movement protein (MP) and coat protein (CP) are expressed from 3'-coterminal subgenomic RNAs (sgRNAs) and deletion analysis suggested that the upper part of this stem-loop, located upstream of the transcription start site, was essential for transcription and that the lower part of the stem had an enhancing role.
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cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts.
TLDR
Sequences of the 200 nucleotides proximal to the 5' termini of four independent cDNA clones were identical to each other and to published sequences, suggesting that independent isolates of TMV may have remarkably similar sequences.
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In planta distribution of 'Candidatus Liberibacter asiaticus' as revealed by polymerase chain reaction (PCR) and real-time PCR.
TLDR
It is found that 'Ca. Liberibacter asiaticus' was distributed in bark tissue, leaf midrib, roots, and different floral and fruit parts, but not in endosperm and embryo, of infected citrus trees, and was transmitted systemically from infection site to different parts of the plant.
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