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Genome size, base composition and karyotype of Jatropha curcas L., an important biofuel plant
TLDR
The genome of J. curcas is relatively small and in the same size range as that of rice, with an average 2C value of 0.85 pg and an average base composition of 38.7% GC.
The polyploidy and its key role in plant breeding
TLDR
The present review provides an up-to-date review concerning from basic issues of polyploidy to aspects regarding the relevance and role of both natural and artificial polyploids in plant breeding programs, progressing to show the revolution promoted by the discovery of natural Polyploidization induction in the breeding program status of distinct crops.
Recovering polyploid papaya in vitro regenerants as screened by flow cytometry
TLDR
Flow cytometry unambiguously evidenced euploid (diploid, mixoploid, triploid and tetraploid) and aneuploid papaya plantlets, indicating that in vitro culture conditions can lead the occurrence of somaclonal variation.
DNA Content Differences Between Male and Female Chicken (Gallus gallus domesticus) Nuclei and Z and W Chromosomes Resolved by Image Cytometry
TLDR
This work was conducted to verify whether the DNA content differences between male and female Gallus gallus domesticus “Leghorn” nuclei and ZZ/ZW chromosomes can be resolved by image cytometry (ICM), and demonstrated resolution power to discriminate lowDNA content differences in genomes.
Assessment of genetic diversity in Psidium guajava L. using different approaches
TLDR
The data obtained for morphology, SSR molecular markers, karyotype and nuclear genome size contributed to expand the knowledge about the genome and genetic diversity of P. guajava, and may aid plant breeders to structure crop improvement programs and contribute to evolutionary approaches.
Revisiting the DNA C-values of the genome size-standards used in plant flow cytometry to choose the “best primary standards”
TLDR
The genome size of Arabidopsis thaliana and other seven plant standards, which were denominated “Doležel’s standard set” and have been widely used in plant DNA measurements, were revisited and Raphanus sativus and Drosophila melanogaster were considered the most inadequate primary standards, whereas A.Thaliana, Solanum lycopersicum and Pisum sativum were found to be the most suitable.
Genome size, base composition and karyotype of Carica papaya L.
TLDR
Flow cytometry and cytogenetic protocols may be useful as a basis for molecular cytogenetics techniques application and made possible to resolve karyogram of hermaphroditic C. papaya.
First Coffea arabica karyogram showing that this species is a true allotetraploid
TLDR
This work suggests that C. arabica is a true non-segmental allotetraploid but originated from different species exhibiting similar and distinct chromosomes.
Comparison of the Coffea canephora and C. arabica karyotype based on chromosomal DNA content
TLDR
The cytogenetic characterization and chromosomal DNA content analyses evidenced that C. canephora is a possible progenitor of C. arabica and is a true allotetraploid originated from a cross between Coffea diploid species.
Flow cytometric analysis using SYBR Green I for genome size estimation in coffee.
TLDR
Evidence is provided that SYBR Green I can be used in flow cytometry measurements of plants, with the advantages of minimizing errors in nuclear DNA content quantification, staining relatively quicker, with high affinity, and being less mutagenic than propidium iodide.
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