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EMAN: semiautomated software for high-resolution single-particle reconstructions.
TLDR
We present EMAN (Electron Micrograph ANalysis), a software package for performing semiautomated single-particle reconstructions from transmission electron micrographs. Expand
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The Structure of ClpB A Molecular Chaperone that Rescues Proteins from an Aggregated State
Molecular chaperones assist protein folding by facilitating their "forward" folding and preventing aggregation. However, once aggregates have formed, these chaperones cannot facilitate proteinExpand
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Cofilin Changes the Twist of F-Actin: Implications for Actin Filament Dynamics and Cellular Function
Cofilin is an actin depolymerizing protein found widely distributed in animals and plants. We have used electron cryomicroscopy and helical reconstruction to identify its binding site on actinExpand
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EMDataBank.org: unified data resource for CryoEM
TLDR
We introduce the Unified Data Resource for CryoEM, a global ‘one-stop shop’ resource for deposition and retrieval of cryoEM maps, models and associated metadata. Expand
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Protein structure fitting and refinement guided by cryo-EM density.
For many macromolecular assemblies, both a cryo-electron microscopy map and atomic structures of its component proteins are available. Here we describe a method for fitting and refining a componentExpand
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Coat protein fold and maturation transition of bacteriophage P22 seen at subnanometer resolutions
Bacteriophage P22 is a prototypical biological machine used for studying protein complex assembly and capsid maturation. Using cryo-EM, we solved the structures of P22 before and after the capsidExpand
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Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions.
Cryo-electron microscopy produces 3D density maps of molecular machines, which consist of various molecular components such as proteins and RNA. Segmentation of individual components in such maps isExpand
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Structure of the AcrAB-TolC multidrug efflux pump
The capacity of numerous bacterial species to tolerate antibiotics and other toxic compounds arises in part from the activity of energy-dependent transporters. In Gram-negative bacteria, many ofExpand
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Outcome of the First Electron Microscopy Validation Task Force Meeting
This Meeting Review describes the proceedings and conclusions from the inaugural meeting of the Electron Microscopy Validation Task Force organized by the Unified Data Resource for 3DEMExpand
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Seeing GroEL at 6 A resolution by single particle electron cryomicroscopy.
We present a reconstruction of native GroEL by electron cryomicroscopy (cryo-EM) and single particle analysis at 6 A resolution. alpha helices are clearly visible and beta sheet density is alsoExpand
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