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The 26S proteasome: a molecular machine designed for controlled proteolysis.
In eukaryotic cells, most proteins in the cytosol and nucleus are degraded via the ubiquitin-proteasome pathway. The 26S proteasome is a 2.5-MDa molecular machine built from approximately 31Expand
A Subcomplex of the Proteasome Regulatory Particle Required for Ubiquitin-Conjugate Degradation and Related to the COP9-Signalosome and eIF3
The proteasome consists of a 20S proteolytic core particle (CP) and a 19S regulatory particle (RP), which selects ubiquitinated substrates for translocation into the CP. An eight-subunit subcomplexExpand
The Proteasome: Paradigm of a Self-Compartmentalizing Protease
The work of one of us (W. B.) was supported by a grant of the Human Frontiers Science Program. We wish to thank Drs. C. P. Hill (Salt Lake City) and J. M. Flanagan (Brookhaven) for making availableExpand
The correlation averaging of a regularly arranged bacterial cell envelope protein
An adaptation of the ‘correlation averaging’ method is described which allows reliable and almost fully automatic image averaging in the case of near‐periodic structures notwithstanding the presenceExpand
Crystal structure of the 20S proteasome from the archaeon T. acidophilum at 3.4 A resolution.
The three-dimensional structure of the proteasome from the archaebacterium Thermoplasma acidophilum has been elucidated by x-ray crystallographic analysis by means of isomorphous replacement andExpand
Formation of cristae and crista junctions in mitochondria depends on antagonism between Fcj1 and Su e/g
Crista junctions (CJs) are important for mitochondrial organization and function, but the molecular basis of their formation and architecture is obscure. We have identified and characterized aExpand
Structural studies by electron tomography: from cells to molecules.
Electron tomography (ET) is uniquely suited to obtain three-dimensional reconstructions of pleomorphic structures, such as cells, organelles or supramolecular assemblies. Although the principles ofExpand
A giant protease with potential to substitute for some functions of the proteasome.
An alanyl-alanyl-phenylalanyl-7-amino-4-methylcoumarin-hydrolyzing protease particle copurifying with 26S proteasomes was isolated and identified as tripeptidyl peptidase II (TPPII), a cytosolicExpand
Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography
Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from DictyosteliumExpand
Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography
Chloroplast function is orchestrated by the organelle's intricate architecture. By combining cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron tomography, we acquiredExpand