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The 26S proteasome: a molecular machine designed for controlled proteolysis.
In eukaryotic cells, most proteins in the cytosol and nucleus are degraded via the ubiquitin-proteasome pathway. The 26S proteasome is a 2.5-MDa molecular machine built from approximately 31…
A Subcomplex of the Proteasome Regulatory Particle Required for Ubiquitin-Conjugate Degradation and Related to the COP9-Signalosome and eIF3
The correlation averaging of a regularly arranged bacterial cell envelope protein
An adaptation of the ‘correlation averaging’ method is described which allows reliable and almost fully automatic image averaging in the case of near‐periodic structures notwithstanding the presence…
The Proteasome: Paradigm of a Self-Compartmentalizing Protease
Crystal structure of the 20S proteasome from the archaeon T. acidophilum at 3.4 A resolution.
The three-dimensional structure of the proteasome from the archaebacterium Thermoplasma acidophilum has been elucidated by x-ray crystallographic analysis by means of isomorphous replacement and…
Formation of cristae and crista junctions in mitochondria depends on antagonism between Fcj1 and Su e/g
A model in which the antagonism between Fcj1 and Su e/g locally modulates the F1FO oligomeric state, thereby controlling membrane curvature of cristae to generate CJs and cristAE tips is proposed.
Structural studies by electron tomography: from cells to molecules.
In combination with advanced computational methods, such as molecular identification based on pattern recognition techniques, cryo-ET is currently the most promising approach to comprehensively map macromolecular architecture inside cellular tomograms.
Architecture of the RNA polymerase II–Mediator core initiation complex
The conserved co-activator complex Mediator enables regulated transcription initiation by RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) comprising all…
Volta potential phase plate for in-focus phase contrast transmission electron microscopy
- R. Danev, B. Buijsse, M. Khoshouei, J. Plitzko, W. Baumeister
- PhysicsProceedings of the National Academy of Sciences
- 20 October 2014
The Volta phase plate has a long service life and has been used for more than 6 mo without noticeable degradation in performance, and the mechanism underlying the VPP is the same as the one responsible for the degradation over time of the performance of thin-film Zernike phase plates, but in the V PP it is used in a constructive way.
A giant protease with potential to substitute for some functions of the proteasome.
TPPII exhibits enhanced activity in proteasome inhibitor-adapted cells and degrades polypeptides by exo- as well as predominantly trypsin-like endoproteolytic cleavage, and may in part account for nonproteasomal epitope generation as postulated for certain major histocompatibility complex class I alleles.