• Publications
  • Influence
Methanogens: reevaluation of a unique biological group.
INTRODUCTION ........ ...... ........ 261 GROWTH OF METHANOGENS 262 Techniques for Growth of Methanogens ..... 262 Principal Media......6..3.. . .... ....... .. . 263 StrainExpand
  • 2,311
  • 274
  • PDF
Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion.
The guanosine triphosphatase Rab1 regulates the transport of newly synthesized proteins from the endoplasmic reticulum to the Golgi apparatus through interaction with effector molecules, but theExpand
  • 441
  • 60
New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere.
  • W. Balch, R. Wolfe
  • Biology, Medicine
  • Applied and environmental microbiology
  • 1 December 1976
The sensitivity of the requirement of Methanobacterium ruminantium strain M1 to a new coenzyme, 2-mercaptoethanesulfonic acid (HS-CoM) was examined by use of new techniques that were developed forExpand
  • 781
  • 53
  • PDF
Adapting Proteostasis for Disease Intervention
The protein components of eukaryotic cells face acute and chronic challenges to their integrity. Eukaryotic protein homeostasis, or proteostasis, enables healthy cell and organismal development andExpand
  • 1,745
  • 52
  • PDF
Functional Amyloid Formation within Mammalian Tissue
Amyloid is a generally insoluble, fibrous cross-β sheet protein aggregate. The process of amyloidogenesis is associated with a variety of neurodegenerative diseases including Alzheimer, Parkinson,Expand
  • 624
  • 36
  • PDF
Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport
COPI and COPII are vesicle coat complexes whose assembly is regulated by the ARF1 and Sar1 GTPases, respectively. We show that COPI and COPII coat complexes are recruited separately and independentlyExpand
  • 399
  • 35
  • PDF
GTP-binding mutants of rab1 and rab2 are potent inhibitors of vesicular transport from the endoplasmic reticulum to the Golgi complex
We have examined the role of ras-related rab proteins in transport from the ER to the Golgi complex in vivo using a vaccinia recombinant T7 RNA polymerase virus to express site-directed rab mutants.Expand
  • 459
  • 34
  • PDF
Hsp90 Cochaperone Aha1 Downregulation Rescues Misfolding of CFTR in Cystic Fibrosis
The pathways that distinguish transport of folded and misfolded cargo through the exocytic (secretory) pathway of eukaryotic cells remain unknown. Using proteomics to assess global cystic fibrosisExpand
  • 532
  • 33
  • PDF
Dominant inhibitory mutants of ARF1 block endoplasmic reticulum to Golgi transport and trigger disassembly of the Golgi apparatus.
Using three different trans dominant mutants of bovine ARF1 affecting GDP exchange or GTP hydrolysis we demonstrate the central role of ARF1 in controlling vesicular traffic from the endoplasmicExpand
  • 391
  • 33
Biological and chemical approaches to diseases of proteostasis deficiency.
Many diseases appear to be caused by the misregulation of protein maintenance. Such diseases of protein homeostasis, or "proteostasis," include loss-of-function diseases (cystic fibrosis) andExpand
  • 893
  • 31
  • PDF