• Publications
  • Influence
An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy
TLDR
It is found that confocal imaging gives greatly enhanced images of biological structures viewed with epifluorescence, and the improvements are such that it is possible to optically section thick specimens with little degradation in the image quality of interior sections.
Replication occurs at discrete foci spaced throughout nuclei replicating in vitro.
TLDR
Analysis of nuclei fixed early in replication reveals that these foci of DNA replication number about 100-300 for each nucleus and probably represent the replicon clusters already described for tissue culture cells, indicating that replication forks remain tightly clustered in groups of at least 300 throughout the period of replication.
Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopy.
TLDR
The fluorescence excitation and emission characteristics of DRAQ5 in living and fixed cells permit the incorporation of the measurement of cellular DNA content into a variety of multiparameter cytometric analyses.
Reversible Mechanochemical Cycle in the Contraction of Vorticella
TLDR
The behaviour of glycerinated preparations was investigated under precisely defined conditions and it was found that contraction was not abolished by ATPase inhibitors.
Evidence for a New Mechanism of Cell Motility
Contraction of the stalks of peritrich ciliates depends on a change of state induced by calcium in a rubber-like polymer, without ATP hydrolysis.
Male mating success and paternity in the grey seal, Halichoerus grypus: a study using DNA fingerprinting
TLDR
Overall, DNA typed males were more dominant, maintained positions amongst the females for longer, and accounted for disproportionately more paternities than untyped males, however, the reproductive success of the typed males is not as great as their behavioural domination of copulatory opportunities would suggest.
Structure and coiling of the stalk in the peritrich ciliates Vorticella and Carchesium.
  • W. Amos
  • Biology
    Journal of cell science
  • 1972
TLDR
Study with the light microscope and the electron microscope suggests that the structure of the tube and the course of the organelle determine the form of the coiling of the stalk of Carcliesium and Vorticella.
Calcium-binding proteins in a vorticellid contractile organelle.
TLDR
In calcium-buffered polyacrylamide gels, it was observed that a reduction in the electrophoretic mobility of the spasmins was induced specifically by calcium (but not magnesium) at the same low concentrations as induce contraction, indicating that the spasms are calcium-binding proteins which may be involved directly in the calcium-induced contraction of thespasmoneme.
Contraction and calcium binding in the vorticellid ciliates.
  • W. Amos
  • Chemistry, Medicine
    Society of General Physiologists series
  • 1975
Molecules of the cytoskeleton
TLDR
This volume describes the properties of the individual proteins of the cytoskeleton and their assembly into the filamentous structures that are responsible for the remarkably stiff consistency of cytoplasm.
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