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Spectrophotometric determination of lipases, lysophospholipases, and phospholipases.
Thioester substrate analog assay procedures are more rapid, sensitive, convenient, continuous, and less expensive than the classical radiochemical techniques. Expand
Ophiobolin A. A natural product inhibitor of calmodulin.
The results of the present study suggest that calmodulin may be one of the target proteins of the phytotoxic action of ophiobolin A and that the interaction of ospinol A with cal modulin may involve a covalent modification of the protein by the fungal metabolite. Expand
Role of calmodulin inhibition in the mode of action of ophiobolin a.
Calmodulin is a target molecule in the root for the toxicity of ophiobolin A, a fungal toxin, which activates the bovine brain calmodulin-dependent cyclic nucleotide phosphodiesterase and has electrophoretic mobility very similar to that of bovines brain cal modulin. Expand
Anti-allergic actions of disodium cromoglycate and other drugs known to inhibit cyclic 3',5'-nucleotide phosphodiesterase.
Disodium cromoglycate (DSCG) inhibited rat peritoneal mast cell degranulation induced by anti-rat serum, noradrenaline, imidazole, phospholipase-A, and antigen. Antigen-induced degranulation was also
Use of calmodulin affinity chromatography for purification of specific calmodulin-dependent enzymes.
This chapter discusses the preparation of the calmodulin (CaM)-affinity column and its general application for the purification of CaM-regulated proteins, which includes CaM/Sepharose 4B affinity chromatography in combination with additional protein purification techniques. Expand
Mast cell degranulation: a comparison of the inhibitory actions of disodium cromoglycate, drugs known to influence the level of intracellular cyclic nucleotide and diisopropylfluorophosphate (DFP).
Rat peritoneal mast cells have been used to study further the effect of drugs on the calcium-independent and calcium-dependent stages of phospholipase-A-induced mast cell degranulation. Evidence is pr
Effect of lung tissue on the inhibitory actions of isoprenaline, theophylline, disodium cromoglycate and PGE1 on antigen-induced mast cell degranulation.
Isoprenaline, disodium cromoglycate and PGE1 poorly inhibit antigen-induced degranulation of sensitised rat peritoneal mast cells, but in the presence of chopped rat lung their inhibitory activitiesExpand
Effect of disodium cromoglycate on various types of anaphylactic reaction in the guinea pig.
The development of allergic reactivity and the effect of disodium cromoglycate (DSCG) on that development were studied in groups of actively sensitized guinea pigs using both ocular sensitivity and an
The anti-anaphylactic actions of disodium cromoglycate, theophylline, isoprenaline and prostaglandins.
Various drugs used in the treatment of either allergy or inflammation have been studied in the rat. Prausnitz-Kustner (PK) type reactions, passive cutaneous anaphylaxis (PCA) and peritoneal mast cell