Effect of benzoic acid on metabolic fluxes in yeasts: A continuous‐culture study on the regulation of respiration and alcoholic fermentation
- C. Verduyn, E. Postma, W. A. Scheffers, J. P. van Dijken
- BiologyYeast
- 1 July 1992
Addition of benzoate to the medium reservoir of glucose‐limited chemostat cultures of Saccharomyces cerevisiae CBS 8066 growing at a dilution rate (D) of 0.10 h−1 resulted in a decrease in the…
Physiology of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures.
- C. Verduyn, E. Postma, W. A. Scheffers, J. Van Dijken
- BiologyJournal of General Microbiology
- 1 March 1990
The physiology of Saccharomyces cerevisiae CBS 8066 was studied in anaerobic glucose-limited chemostat cultures in a mineral medium supplemented with ergosterol and Tween 80, suggesting that the observed difference in cell yield may be ascribed to an uncoupling effect of acetic acid.
Enzymic analysis of the crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae
- E. Postma, C. Verduyn, W. A. Scheffers, J. Van Dijken
- BiologyApplied and Environmental Microbiology
- 1 February 1989
The physiology of Saccharomyces cerevisiae CBS 8066 was studied in glucose-limited chemostat cultures and it was concluded that the occurrence of alcoholic fermentation is not primarily due to a limited respiratory capacity, but rather, organic acids produced by the organism may have an uncoupling effect on its respiration.
Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status
- A. Maris, D. A. Abbott, J. Pronk
- Engineering, BiologyAntonie van Leeuwenhoek
- 11 October 2006
The current status with respect to alcoholic fermentation of the main plant biomass-derived monosaccharides by this yeast is reviewed and possible approaches for metabolic engineering of galacturonic acid and rhamnose fermentation by S. cerevisiae are discussed.
Properties of the NAD(P)H-dependent xylose reductase from the xylose-fermenting yeast Pichia stipitis.
- C. Verduyn, R. V. van Kleef, J. Frank, H. Schreuder, J. Van Dijken, W. A. Scheffers
- Biology, EngineeringBiochemical Journal
- 15 March 1985
Xylose reductase from the xylose-fermenting yeast Pichia stipitis was purified to electrophoretic and spectral homogeneity via ion-exchange, affinity and high-performance gel chromatography and is an aldose reducase (EC 1.1.21).
Redox balances in the metabolism of sugars by yeasts (NAD(H); NADP(H); glucose metabolism; xylose fermentation; ethanol; Crabtree effect; Custers effect)
- J. P. Dijken, W. A. Scheffers
- Biology
- 7 January 1986
1. SUMMARY The central role of the redox couples NAD+/ NADH and NADP+/NADPH in the metabolism of sugars by yeasts is discussed in relation to energy metabolism and product formation. Besides their…
A theoretical evaluation of growth yields of yeasts
- C. Verduyn, A. H. Stouthamer, W. A. Scheffers, J. P. Dijken
- BiologyAntonie van Leeuwenhoek
- 2004
Calculations for growth of both yeasts on glucose, ethanol, and acetate made clear that only by assuming a fixed difference between theoretical and experimental ATP requirements, the P/O-ratios are more or less independent of the growth substrate.
Oxygen requirements of yeasts
- W. Visser, W. A. Scheffers, W. H. Batenburg-van der Vegte, J. Van Dijken
- BiologyApplied and Environmental Microbiology
- 1 December 1990
Type species of 75 yeast genera examined for their ability to grow anaerobically in complex and mineral media revealed that Saccharomyces cerevisiae stands out as a yeast capable of rapid growth at low redox potentials.
Energetics of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures.
- C. Verduyn, E. Postma, W. A. Scheffers, J. Van Dijken
- Biology, EngineeringJournal of General Microbiology
- 1 March 1990
The energetics of Saccharomyces cerevisiae were studied in anaerobic glucose-limited chemostat cultures via an analysis of biomass and metabolite production and a linear correlation was found between the energy required to compensate for import of protons and the amount of acid added.
The role of redox balances in the anaerobic fermentation of xylose by yeasts
- P. M. Bruinenberg, Peter V. M. Bot, J. P. Dijken, W. A. Scheffers
- Biology, EngineeringEuropean Journal of Applied Microbiology and…
- 1 September 1983
A hypothesis is put forward which explains that ethanol production from xylose by yeast under anaerobic conditions is negligible, and it is suggested that the failure to fermentxylose anaerobically is due to a discrepancy between production and consumption of NADH in the overall conversion of xylOSE to ethanol.
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