W. Walter Lorenz

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Conifers comprise an ancient and widespread plant lineage of enormous commercial and ecological value. However, compared to model woody angiosperms, such as Populus and Eucalyptus, our understanding of conifers remains quite limited at a genomic level. Large genome sizes (10,000–40,000 Mbp) and large amounts of repetitive DNA have limited efforts to produce(More)
PtGen2 is a 26,496 feature cDNA microarray containing amplified loblolly pine ESTs. The array is produced in our laboratory for use by researchers studying gene expression in pine and other conifer species. PtGen2 was developed as a result of our gene discovery efforts in loblolly pine, and is comprised of sequences identified primarily from root tissues,(More)
Tissues isolated from conifer species, particularly those belonging to the Pinaceae family, such as loblolly pine (Pinus taeda L.), contain high concentrations of phenolic compounds and polysaccharides that interfere with RNA purification. Isolation of high-quality RNA from these species requires rigorous tissue collection procedures in the field and the(More)
Phenylalanine ammonia lyase (PAL) is a key enzyme of the phenylpropanoid pathway. PAL genes are less extensively studied in gymnosperms than angiosperms. Our interest in the PAL genes in Pinus taeda stems from their potential role in pine defense mechanisms. Despite the number of characterized gymnosperm PAL genes, the functional diversity of these and the(More)
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