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The calcium indicator fura-2 was used to study the effect of hypotonic solutions on the intracellular calcium concentration, [Ca(2+)](i), in a human osteoblast-like cell line. Decreasing the tonicity of the extracellular solution to 50% leads to an increase in [Ca(2+)](i) from approximately 150 nm up to 1.3 microm. This increase in [Ca(2+)](i) was mainly(More)
AIM OF THE STUDY It has been well shown by human and animal studies that mechanical load is an important regulator of skeletal mass and architecture. However, cellular reactions which adapt bone tissue to the mechanical environment are not definitively determined. For this purpose we studied the cell activity of human bone derived cell cultures after(More)
Enamel of human permanent teeth was sectioned and ground with 2 planes perpendicular to each other extending centrifugally from the dentino-enamel-junction to the crown surface. Prisms were made visible by acid etching before evaluation under the SEM. In the vicinity of the dentino-enamel-junction more prisms were found to be cut longitudinally, while close(More)
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