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The effect of insulin in vitro on the fluidity of the human erythrocyte membrane in Type I (insulin-dependent) diabetic patients and healthy control subjects was investigated using a fluorescence technique. It was found that the addition of 10-9 mol/l porcine insulin significantly increased fluorescent probe lateral mobility in the membrane lipid layer but(More)
Erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity were estimated after in vitro combined treatment of human erythrocytes with perindoprilat and low-power red light irradiation. Membrane fluidity was determined using fluorescent labels spectroscopy; membrane potential was evaluated by means of potential-sensitive fluorescent(More)
Mittels der Spinprobenmethode wurden die Veränderungen der Mikroviskosität von dichtgetrennten Rindererythrozyten untersucht. Die Verlängerung der Rotations-Korrelationszeit von Tempamine als einer Spinprobe belegt den Anstieg der Mikroviskosität im Erythrozyteninneren bei der in vivo-Alterung. Dieser Anstieg ist wahrscheinlich von Veränderungen des(More)
Spin-label studies of the effects of hyperthermia on the erythrocyte membrane revealed a decrease in the fluidity of lipids and changes in the state of membrane proteins. The rate of haemolysis in iso-osmotic glycerol solution was increased. Changes of most of the parameters studied when plotted in Arrhenius coordinates revealed a discontinuity (critical(More)
Gamma-irradiation of bovine erythrocyte membranes (0.1-4 Mrad) resulted in a decrease in the degree of order of membrane lipids, as measured by spin-labelled fatty acid esters, at the depth of C12 but not at the depth of C5. Dose dependence of this phenomenon corresponded to dose dependence of malondialdehyde formation in the membranes. On this basis a(More)
The interaction of propranolol with model phospholipid membranes was studied using various experimental techniques. The partition coefficient of propranolol in the negatively charged membranes of vesicles prepared from phosphatidylserine and phosphatidic acid was found to be more than 20-times higher than in neutral phosphatidylcholine membranes.(More)