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A fundamental step in the replication of a viral particle is the self-assembly of its rigid shell (capsid) from its constituent proteins. Capsids play a vital role in genome replication and intercellular movement of viruses, and as such, understanding viral assembly has great potential in the development of new antiviral therapies and a systematic treatment(More)
Recent experiments show that, in the range from approximately 15 to 45 degrees C, an increase in the temperature promotes the spontaneous assembly into capsids of the Escherichia coli-expressed coat proteins of hepatitis B virus. Within that temperature interval, an increase in ionic strength up to five times that of standard physiological conditions also(More)
We report a single step procedure to prepare monodisperse colloidal poly(methyl)methacrylate (PMMA) particles where fluorescent dyes are incorporated into the polymer network. The particles are sterically stabilized against flocculation by poly(12-hydroxystearic acid). The fluorescent dyes are RITC (rhodamine isothiocyanate)-aminostyrene (RAS) and(More)
We present a statistical mechanical model based on the principle of mass action that explains the main features of the in vitro aggregation behavior of the coat protein of tobacco mosaic virus (TMV). By comparing our model to experimentally obtained stability diagrams, titration experiments, and calorimetric data, we pin down three competing factors that(More)
The depletion-induced phase separation in a mixture of colloidal particles (PMMA-latex) and nonadsorbing polymers [poly(styrene)] in a solvent (mixture of tetralin, cis-decalin, and carbon tetrachloride) was investigated in real space with confocal scanning laser microscopy in the initial, intermediate, and final stage. It was found that the kinetics and(More)
Colloidal particles with site-specific directional interactions, so called "patchy particles", are promising candidates for bottom-up assembly routes towards complex structures with rationally designed properties. Here we present an experimental realization of patchy colloidal particles based on material independent depletion interaction and surface(More)
Confocal scanning laser microscopy has been used to quantitatively analyze the structure and dynamics of concentrated suspensions of spherical colloids in which the magnitude of the short-range attractive potential is increased by adding nonadsorbing polymers. These systems undergo a reentrant glass transition upon increasing polymer concentration. We find(More)
The influence of gravity on the long-time behavior of the mean squared displacement in glasses of polydisperse colloidal hard spheres was studied by means of real-space fluorescent recovery after photobleaching. We present, for the first time, a significant influence of gravity on the mean squared displacements of the particles. In particular, we observe(More)
We report the direct measurement of thermodynamic properties of colloidal hard spheres by optical microscopy. By using confocal microscopy, we obtain three-dimensional snapshots of a colloidal hard-sphere suspension over a wide range of densities. From these snapshots, the available volume to insert an additional sphere and the surface area of that volume(More)