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We tested the hypothesis that somatostatin (SRIF) inhibits insulin secretion from an SV40 transformed hamster beta cell line (HIT cells) by an effect on the voltage-dependent Ca2+ channels and examined whether G-proteins were involved in the process. Ca2+ currents were recorded by the whole cell patch-clamp method, the free cytosolic calcium, [Ca2+]i, was(More)
We present a method to document ciliary beat frequency with the linescan function of a scanning confocal microscope, using ciliated tracheal cells and free-swimming rotifers as examples. Depending on the clarity of the original data, the ciliary beat frequency can be determined from the confocal linescan directly or from an intensity linescan analysis of(More)
Acknowledgement I would like to dedicate this thesis to my parents. This work could never be accomplished without their support. i Abstract As the Internet becomes more and more populous, peopleconcern more about the copyright protection issue for digital data such as images and audio. Digital watermarking technique can hide data in images or audio to(More)
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