1. A method is given for the concentration and purification of the soluble specific substance of the pneumococcus. 2. The material obtained by this method is shown to consist mainly of a carbohydrate which appears to be a polysaccharide built up of glucose molecules. 3. Whether the soluble specific substance is actually the polysaccharide, or occurs merely… (More)
The soluble specific substance of Pneumococcus Type I has been chemically isolated from the bacterial cells and from autolyzed cultures as an acetyl polysaccharide. So far as could be determined by the methods employed, the acetyl polysaccharide in highly purified form absorbs from Type I antipneumococcus serum all demonstrable type-specific precipitins,… (More)
Pneumococci contain a non-protein constituent which, on the basis of its chemical and immunological properties, appears to be a carbohydrate distinct from the type-specific carbohydrate and common to the species.
The genes coding for the 5S rRNA and another stable RNA, termed 7S RNA, in Halobacterium halobium were isolated from a genomic library of this archaebacterium and their nucleotide sequences determined. Both genes are colinear with their transcripts (5S rRNA and 7S RNA), but 5S rRNA and possibly also 7S RNA isolated from other halobacteria carry additional… (More)
1. By partial acid hydrolysis a specific carbohydrate may be isolated from gum arabic (gum acacia). This carbohydrate is comparable in its precipitating activity for Type II (and Type III) antipneumococcus serum with the bacterial soluble specific substances themselves. 2. On hydrolysis this fraction yields galactose and two or more complex sugar acids, one… (More)
1. A method is given for the isolation of a specifically reacting nitrogen-free polysaccharide from the so called E strain of Friedländer's bacillus. 2. The properties of this polysaccharide are described.
1. Autolysis of Pneumococcus is accompanied by proteolysis, which results in an increase in amino and non-coagulable nitrogen. 2. Autolysis of Pneumococcus is accompanied by lipolysis during which there is a liberation of ether-soluble fatty acids. 3. When extracts containing the active intracellular enzymes are added to heat-killed pneumococci, lysis of… (More)
1. Methods are given for the isolation of specifically reacting nitrogen-free polysaccharides from Type A and Type C Friedländer's bacillus. 2. The properties of these specific carbohydrates have been outlined.
1. Methods for the concentration of Type I pneumococcus antibody have been outlined. 2. The physical and chemical properties of the purified antibody have been described. 3. The chemical basis of serological specificity has been discussed.