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Antagonistic Pseudomonas spp. (DF-41 and PA-23) were evaluated for inhibition of germination of ascospores, and for the effect of timing of application and its effect on biological control of Sclerotinia sclerotiorum (Lib.) de Bary, causal agent of stem rot of canola. Population dynamics were also assessed. In all studies, a petal inoculation technique was(More)
Blackleg, also known as Phoma stem canker, caused by Leptosphaeria maculans (Phoma lingam), is one of the most serious diseases of canola worldwide. In this study, the mean disease severity (Ds) and incidence (Di) of canola cv. Westar plants infected at the cotyledon, three-leaf, and six-leaf stages by pycnidiospores of L. maculans were monitored in the(More)
Total RNA from wheat spikes infected with F. graminearum Fg2 was extracted and the mRNA was purified. Switching Mechanism at 5' end of the RNA Transcript (SMART) technique and CDS Ill/3' primer were used for first-strand cDNA synthesis using reverse transcriptase by RT-PCR. Primer extension polymerase chain reaction was used to construct the double-strand(More)
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