W. G. Dilantha Fernando

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Pseudomonas chlororaphis PA23 is a biocontrol agent that protects against the fungal pathogen Sclerotinia sclerotiorum. Employing transposon mutagenesis, we isolated a gacS mutant that no longer exhibited antifungal activity. Pseudomonas chlororaphis PA23 was previously reported to produce the nonvolatile antibiotics phenazine 1-carboxylic acid and(More)
Bacteria, isolated from canola and soybean plants, produced antifungal organic volatile compounds. These compounds inhibited sclerotia and ascospore germination, and mycelial growth of Sclerotinia sclerotiorum, in vitro and in soil tests. Ascospore germination in cavity slides was inhibited 54–90% by the volatile producers. When mycelial plugs or the(More)
Antagonistic Pseudomonas spp. (DF-41 and PA-23) were evaluated for inhibition of germination of ascospores, and for the effect of timing of application and its effect on biological control of Sclerotinia sclerotiorum (Lib.) de Bary, causal agent of stem rot of canola. Population dynamics were also assessed. In all studies, a petal inoculation technique was(More)
Pseudomonas chlororaphis PA23, Pseudomonas spp. strain DF41, and Bacillus amyloliquefaciens BS6 consistently inhibit infection of canola petals by Sclerotinia sclerotiorum in both greenhouse and field experiments. Bacillus thuringiensis BS8, Bacillus cereus L, and Bacillus mycoides S have shown significant inhibition against S. sclerotiorum on plate assays.(More)
Blackleg, caused by Leptosphaeria maculans, is an economically important disease of canola (Brassica napus). Little is known about the current distribution of pathogenicity groups (PGs) of L. maculans in Canada and the United States. Four hundred and eighty-nine isolates of L. maculans or Leptosphaeria biglobosa from western Canada and North Dakota, United(More)
It has been debated how different farming systems influence the composition of soil bacterial communities, which are crucial for maintaining soil health. In this research, we applied high-throughput pyrosequencing of V1 to V3 regions of bacterial 16S rRNA genes to gain further insight into how organic and conventional farming systems and crop rotation(More)
Canola (Brassica napus L. and B. rapa L.), is one of the major oilseed crops grown worldwide. With significant expansion of acreage sown to canola during the past two decades, concern about blackleg, the most serious disease of canola, has increased (3,4). Blackleg disease is caused by Leptosphaeria maculans (Desmaz.) Ces. & De Not. (anamorph: Phoma lingam(More)
ABSTRACT The spread of Fusarium head blight of wheat from a small area inoculum source was examined in wheat plots (100, 625, or 2,500 m(2)) inoculated in the center with Gibberella zeae-colonized corn kernels or macro-conidia sprayed on heads at anthesis. With the first inoculation method, disease foci were produced from ascospores released from perithecia(More)
Biological control of Sclerotinia sclerotiorum (Lib.) de Bary by Pseudomonas and Bacillus species on canola petals Abstract Four bacterial strains, Pseudomonas chlororaphis (PA-23), Bacillus amyloliquefaciens (BS6), Pseudomonas sp. (DF41) and B. amyloliquefaciens (E16) which had been found to have biocontrol activity in vitro assays against Sclerotinia(More)
  • M U Salam, L Fitt, J.-N Aubertot, A J Diggle, Y J Huang, M J Barbetti +8 others
  • 2007
Weather-based models (Improved Blackleg Sporacle and SporacleEzy) to predict the date of onset of seasonal release from oilseed rape debris of ascospores of Leptosphaeria maculans or L. biglobosa , causes of phoma stem canker, were developed and tested with data from diverse environments in Australia, Canada, France, Poland and the UK. Parameters were(More)