W. F. Gross

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A novel acyltransferase from cotyledons of tomato (Lycopersicon esculentum Mill.), which catalyzes the transfer of caffeic acid from chlorogenic acid (5-O-caffeoylquinic acid) to glucaric and galactaric acids, was purified with a 2400-fold enrichment and a 4% recovery. The enzyme showed specific activities (theoretical V(max) per milligram of protein) of(More)
The phenylpropane metabolism of tomato (Lycopersicon esculentum Mill) cotyledons was investigated. The HPLC analysis revealed two hydroxycinnamic-acid conjugates as major components, identified as chlorogenic acid (5-O-caffeoylquinic acid) and caffeoylglucaric acid (2-O- or 5-O-caffeoyl-glucaric acid). Quantitative analyses indicated a precursor-product(More)
The intracellular distribution of enzymes capable of catalyzing the reactions from phosphoglycolate to glycerate in the bluegreen colored eucaryotic alga Cyanidium caldarium has been studied. After separating the organelles from a crude homogenate on a linear flotation gradient, the enzymes glycolate oxidase and glutamate-glyoxylate aminotransferase along(More)
The intracellular distribution of enzymes, participating in the beta-oxidation of fatty acids in the eucaryotic alga Cyanidium has been studied. After separating the organelles from a crude homogenate on a linear flotation gradient, the enzymes enoyl-CoA hydratase, hydroxyacyl-CoA dehydrogenase, and thiolase were present in the mitochondrial fraction(More)
Mit den b e k a n n t e n M e t hoden [ t 3 ] der M e h r k o m p o n e n t e n ana lyse lassen sich h ie raus die IZonzentrazionen x'i berechnen. Auf3erdem k a n n mail m i t Hilfe der b e k a n n t e n K o m p o n e n t e xoso(2 ) u n d den gemessenen Spek t ren E*(2) u n d E ( ) 0 den L ich tweg als E* (~) -E (Z) d(~) (5) x0 eo (Z) berechnen. I m(More)