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BACKGROUND Previous systems for dot (signal) counting in fluorescence in situ hybridization (FISH) images have relied on an auto-focusing method for obtaining a clearly defined image. Because signals are distributed in three dimensions within the nucleus and artifacts such as debris and background fluorescence can attract the focusing method, valid signals(More)
—Fast and accurate analysis of fluorescence in-situ hy-bridization (FISH) images for signal counting will depend mainly upon two components: a classifier to discriminate between artifacts and valid signals of several fluorophores (colors), and well discriminating features to represent the signals. Our previous work has focused on the first component. To(More)