W. E. Magee

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Nodules from leguminous plants will continue to fix N2 into acid extractable compounds for several hours after they have been excised from the roots. The capacity of nodules to fix N2 is decreased twoto five-fold by slicing and is abolished by grinding the nodules (2). The decrease in nitrogen fixation which accompanies slicing of the nodules and suspending(More)
Virulent serotypes of Yersinia enterocolitica carry a plasmid (pYV) encoding a family of proteins that are released into the medium and whose expression is temperature and calcium regulated. The plasmid is easily lost from cells during their growth in the laboratory. We have used sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting(More)
Cultivation of a model hybridoma cell line in Dulbecco's modification of Eagle's minimal essential medium (DMEM) at various glucose and glutamine levels showed that maxmium cell growth was obtained in a broad range of glucose/glutamine ratios of 0.6 to 6.0. A new parameter called the viability index is proposed to quantify cell viability. A strong(More)
Cell viability was improved by supplemental feeding of amino acids and vitamins in batch culture of hybridoma cells. Cells could be maintained over a 10 day period following exponential growth at a constant viable cell concentration of 2.1×106 cells/ml. Concentrations of monoclonal antibody (MAb) reached 140 mg/l, a value of nearly four times that found in(More)
Ammonia is generally conceded to be the "key intermediate" in biological nitrogen fixation-that is, the compound which reacts to form the first stable organic compound, glutamic acid (Wilson and Burris, 1953). However, there is virtually no information concerning the reactions functioning in the conversion of N2 to ammonia. Reproducible nitrogen fixation by(More)
Nodules from leguminous plants will continue to fix N2 into acid extractable compounds for several hours after they have been excised from the roots. The capacity of nodules to fix N2 is decreased twoto five-fold by slicing and is abolished by grinding the nodules (2). The decrease in nitrogen fixation which accompanies slicing of the nodules and suspending(More)
Effect of glucose concentration and pH on lactic acid formation was investigated in batch cultures of a hybridoma cell line. Lactate formation increases with growth rate. High glucose concentration leads to extensive lactate formation only during growth phase and not during stationary phase. Lactate formation also may serve to regulate extracellular pH to(More)